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Team:Calgary/13 July 2009
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| - | + | Gel Extraction of <i>luxCDABE</i> | |
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| - | + | * The main purpose of gel extraction is to ensure that only <i>luxCDABE</i> is present for cloning. The problem with cloning <i>luxCDABE</i> is that we are cloning in smaller pieces of DNA, rather than the gene of interest. | |
| + | * Prepared 0.7% gel and ran the gel for 90 minutes at 90V. | ||
| + | * Followed QIAquick Gel Extraction Kit Protocol (please see protocol page for details) | ||
| + | * The concentrations that we ended up getting from the gel extraction is low. We re-ran the 0.7% gel with a larger amount of product, however, the kit is only optimal for 3-4 kbs. | ||
| + | * Gradient PCR of <i>luxCDABE</i> with the biobrick sites at the end. (We ran out of PCR products to work with) | ||
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Revision as of 00:39, 1 August 2009
UNIVERSITY OF CALGARY
