Template:Team:KULeuven/3 August 2009/VanillinProduction
From 2009.igem.org
(Difference between revisions)
(New page: We received the ComT, Sam5 and Sam8 from Edinburgh today. They were put in competent cells by electroporation. * Sam5 + RBS in pSB1A2 (with PstI site removed) * Sam8 + RBS in pSB1A2 * Sam...) |
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Line 3: | Line 3: | ||
* Sam5 + RBS in pSB1A2 (with PstI site removed) | * Sam5 + RBS in pSB1A2 (with PstI site removed) | ||
* Sam8 + RBS in pSB1A2 | * Sam8 + RBS in pSB1A2 | ||
- | * Sam8 in pSB1A2 coding sequence {{kulpart|BBa_I742142}} | + | * Sam8 in pSB1A2 coding sequence, {{kulpart|BBa_I742142}} |
- | * ComT in pSB1A2 coding sequence {{kulpart|BBa_I742107}} | + | * ComT in pSB1A2 coding sequence, {{kulpart|BBa_I742107}} |
The cells were put in liquid LB to recuperate from the procedure and transfered onto LB/amp plates and grown overnight at 37°C. | The cells were put in liquid LB to recuperate from the procedure and transfered onto LB/amp plates and grown overnight at 37°C. |
Revision as of 08:25, 4 August 2009
We received the ComT, Sam5 and Sam8 from Edinburgh today. They were put in competent cells by electroporation.
- Sam5 + RBS in pSB1A2 (with PstI site removed)
- Sam8 + RBS in pSB1A2
- Sam8 in pSB1A2 coding sequence,
- ComT in pSB1A2 coding sequence,
The cells were put in liquid LB to recuperate from the procedure and transfered onto LB/amp plates and grown overnight at 37°C.