Uppsala-Sweden/3 August 2009

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(→Glycerol Stock for Z. Mobilis)
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--[[User:Karl.brune|Karl.brune]] 12:42, 3 August 2009 (UTC)
--[[User:Karl.brune|Karl.brune]] 12:42, 3 August 2009 (UTC)
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==Buffers for transformation==
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We prepared the buffers (TE and SOB) needed for the transformation.
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The protocols can be found here [http://openwetware.org/wiki/SOB] and [http://openwetware.org/wiki/TE_buffer]
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--[[User:Flormane|Flormane]] 12:13, 4 August 2009 (UTC)
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==PCR, Kivd, ADH2 and PpetE==
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We started a PCR reaction to extract the Kivd gene from Lactoctococcus Lactis sp.Lactis, ADH2 from Saccharomyces cerevisiae and the promoter PpetE from Synechocystis.
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The primers used was [[Media:primers_090803_adh2_kivd_ppete.xls]]
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--[[User:Flormane|Flormane]] 12:13, 4 August 2009 (UTC)

Revision as of 12:13, 4 August 2009

Glycerol Stock for Z. Mobilis

The test protocol works really fine as prelimary test results show. Thus I made final gylcerol stocks for Z. Mobilis.

The protocol can be found here: Media:Glycerol_Stock_Z_mobilis.xls

--Karl.brune 12:42, 3 August 2009 (UTC)

Buffers for transformation

We prepared the buffers (TE and SOB) needed for the transformation.

The protocols can be found here [http://openwetware.org/wiki/SOB] and [http://openwetware.org/wiki/TE_buffer] --Flormane 12:13, 4 August 2009 (UTC)

PCR, Kivd, ADH2 and PpetE

We started a PCR reaction to extract the Kivd gene from Lactoctococcus Lactis sp.Lactis, ADH2 from Saccharomyces cerevisiae and the promoter PpetE from Synechocystis.

The primers used was Media:primers_090803_adh2_kivd_ppete.xls --Flormane 12:13, 4 August 2009 (UTC)

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