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Uppsala-Sweden/4 August 2009
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==Butanol Evolution== | ==Butanol Evolution== | ||
26ml flasks from the Butanol recovery were supplied with isobutanol regarding their former concentration. (0,25% | 0,25% | 0,5% | control w/o). Flask were incubated in 30°C growth room on 120rpm shaker. | 26ml flasks from the Butanol recovery were supplied with isobutanol regarding their former concentration. (0,25% | 0,25% | 0,5% | control w/o). Flask were incubated in 30°C growth room on 120rpm shaker. | ||
| + | |||
| + | ==PCR (Kivd, ADH2 and PpetE) and Gel run== | ||
| + | This is continued since yesterday. | ||
| + | |||
| + | We ran the PCR products on a gel with the following setup and result. | ||
| + | |||
| + | Setup: | ||
| + | [[Media:gel_run_090804_adh2_kivd_ppete.xls]] | ||
| + | |||
| + | Result: | ||
| + | [[Image:20090804_2 RT-PCR alr2938F-R no,33-40.jpg]] | ||
| + | --[[User:Flormane|Flormane]] 12:32, 4 August 2009 (UTC) | ||
Revision as of 12:32, 4 August 2009
Ethanol Evolution
1,15ml of 95 EtOH were added to a 26ml flask from the recovery protocol of the ethanol evolution project. Thus resulting in approx. 4% EtOH concentration (0,03981% in theory) but I expect that some water evaporated during the culturing thus the former volume (26ml) was decreased. Flask were incubated in 30°C growth room on 120rpm shaker.
Butanol Evolution
26ml flasks from the Butanol recovery were supplied with isobutanol regarding their former concentration. (0,25% | 0,25% | 0,5% | control w/o). Flask were incubated in 30°C growth room on 120rpm shaker.
PCR (Kivd, ADH2 and PpetE) and Gel run
This is continued since yesterday.
We ran the PCR products on a gel with the following setup and result.
Setup: Media:gel_run_090804_adh2_kivd_ppete.xls
Result:
--Flormane 12:32, 4 August 2009 (UTC)
