From 2009.igem.org
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| + | Pqrr4+I13500(RBS+GFP) was constructed to verify the Mutants; however, we realized that both of our parts were in high copy plasmids, which a single cell can take one of. Thus the smaller Pqrr4+I13500 circuit is chosen to be plasmid switched into another vector (pSB2K3), which was obtained from the Q04510 (inverter). Today, both the Pqrr4+13500 and pSB2K3 were cut at xbaI+PstI via restriction digest, and it is going to be left in the waterbath(37˚C) overnight. |
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Revision as of 22:00, 4 August 2009
University of Calgary
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CAROL
Modelling Meeting
- No lab experiments performed today (have to re-consider methods) - no colonies were found from the transformation that occured yesterday morning.
- For the modelling meeting, the following points were discussed:
1. A better organization of our work is required. We have both characterization and simulation from the mathematical team, but the work seems unlinked and not organized. Will have a more organized report for next week.
2. Greater detail was explained to us by Thane Kubik regarding the biochemical details that are behind the signalling pathway.
3. We need to start thinking about how we are going to present our two modelling system that we have created.
4. We will meet again on thursday and Afshin will give us a better overview of membrane computing.
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CHINMOYEE
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EMILY
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FAHD
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IMAN
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JAMIE
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JEREMY
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KATIE
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KEVIN
Preparation for Plasmid switch of Pqrr4+I13500
Pqrr4+I13500(RBS+GFP) was constructed to verify the Mutants; however, we realized that both of our parts were in high copy plasmids, which a single cell can take one of. Thus the smaller Pqrr4+I13500 circuit is chosen to be plasmid switched into another vector (pSB2K3), which was obtained from the Q04510 (inverter). Today, both the Pqrr4+13500 and pSB2K3 were cut at xbaI+PstI via restriction digest, and it is going to be left in the waterbath(37˚C) overnight.
Preparation for Plasmid switch of Pqrr4+I13500
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MANDY
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PATRICK
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PRIMA
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STEFAN
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VICKI
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WIKI CODING HERE
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