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Team:Calgary/4 August 2009
From 2009.igem.org
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<i>Pqrr4</i>+I13500(RBS+GFP) was constructed to verify the Mutants; however, we realized that both of our parts were in high copy plasmids, which a single cell can take one of. Thus the smaller <i>Pqrr4</i>+I13500 circuit is chosen to be plasmid switched into another vector (pSB2K3), which was obtained from the Q04510 (inverter). Today, both the Pqrr4+13500 and pSB2K3 were cut at xbaI+PstI via restriction digest, and it is going to be left in the waterbath(37˚C) overnight. | <i>Pqrr4</i>+I13500(RBS+GFP) was constructed to verify the Mutants; however, we realized that both of our parts were in high copy plasmids, which a single cell can take one of. Thus the smaller <i>Pqrr4</i>+I13500 circuit is chosen to be plasmid switched into another vector (pSB2K3), which was obtained from the Q04510 (inverter). Today, both the Pqrr4+13500 and pSB2K3 were cut at xbaI+PstI via restriction digest, and it is going to be left in the waterbath(37˚C) overnight. | ||
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Overnight cultures of our reporter circuit | Overnight cultures of our reporter circuit | ||
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In order to further verify our reporter circuit (<i>Pqrr4</i>+B0034+K082003 Colony 9) using restriction digest, an overnight culture needs to be grown; thus 2 were grown and are to be shaken overnight at 37˚C | In order to further verify our reporter circuit (<i>Pqrr4</i>+B0034+K082003 Colony 9) using restriction digest, an overnight culture needs to be grown; thus 2 were grown and are to be shaken overnight at 37˚C | ||
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Modelling meeting | Modelling meeting | ||
Revision as of 22:32, 4 August 2009
UNIVERSITY OF CALGARY
