Virginia Commonwealth/5 August 2009
From 2009.igem.org
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[[User:Trentay|Trentay]] 13:19, 5 August 2009 (UTC) | [[User:Trentay|Trentay]] 13:19, 5 August 2009 (UTC) | ||
''Kevin & Adam'' | ''Kevin & Adam'' | ||
+ | *Digest and run gel on backbone | ||
*Digest synthetic promoters and ligate them into backbone with RET | *Digest synthetic promoters and ligate them into backbone with RET | ||
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Revision as of 14:29, 5 August 2009
Contents |
Wednesday 5 August 2009
Results
Maria and Afton
- Transformation Results
Name | Plate | Growth Observation | |
---|---|---|---|
pSB1C3 | CM | 5 small colonies | |
(+) Control | LB | lawn | |
(+) Control zapped | LB | lawn | |
(-) Control | CM | no growth |
Trentay 13:19, 5 August 2009 (UTC)
Tasks
Maria and Afton
- Pick colonies of pSB1C3 w/ P1010 for overnight culture
- Make overnight culture of pSB4C5 and I1352 from frozen stocks
Trentay 13:19, 5 August 2009 (UTC) Kevin & Adam
- Digest and run gel on backbone
- Digest synthetic promoters and ligate them into backbone with RET
Wetlab
Maria and Afton
- 1
- 2
Trentay 13:19, 5 August 2009 (UTC)