Virginia Commonwealth/5 August 2009

From 2009.igem.org

(Difference between revisions)
(Tasks)
(Tasks)
Line 28: Line 28:
[[User:Trentay|Trentay]] 13:19, 5 August 2009 (UTC)
[[User:Trentay|Trentay]] 13:19, 5 August 2009 (UTC)
''Kevin & Adam''
''Kevin & Adam''
 +
*Digest and run gel on backbone
*Digest synthetic promoters and ligate them into backbone with RET
*Digest synthetic promoters and ligate them into backbone with RET
----
----

Revision as of 14:29, 5 August 2009

Contents

Wednesday 5 August 2009

Results

Maria and Afton

  • Transformation Results
Name Plate Growth Observation
pSB1C3 CM 5 small colonies
(+) Control LB lawn
(+) Control zapped LB lawn
(-) Control CM no growth

Trentay 13:19, 5 August 2009 (UTC)


Tasks

Maria and Afton

  • Pick colonies of pSB1C3 w/ P1010 for overnight culture
  • Make overnight culture of pSB4C5 and I1352 from frozen stocks

Trentay 13:19, 5 August 2009 (UTC) Kevin & Adam

  • Digest and run gel on backbone
  • Digest synthetic promoters and ligate them into backbone with RET

Wetlab

Maria and Afton

  • 1
  • 2

Trentay 13:19, 5 August 2009 (UTC)