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Virginia Commonwealth/7 July 2009
From 2009.igem.org
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''Afton and Maria'' | ''Afton and Maria'' | ||
* Digestion | * Digestion | ||
| - | |||
* Agarose Gel preparation/Electrophoresis test | * Agarose Gel preparation/Electrophoresis test | ||
| + | * Spectrometry of Miniprepped DNA | ||
| + | {| cellpadding="5" border="1" padding="5" | ||
| + | |+ | ||
| + | |- | ||
| + | ! Part No.!! A260 (nm) !! A280 (nm) !! A260/A280 !! Volume (µL) !! Conc.(µg/mL) !!Amount (µg) | ||
| + | |- | ||
| + | ! pSB3K3 (1) | ||
| + | | 0.011 || 0.010 || 1.10 || 35 || 11 || 0.385 | ||
| + | |- | ||
| + | ! pSB3K3 (2) | ||
| + | | 0.010 || 0.008 || 1.25 || 35 || 10 || 0.35 | ||
| + | |- | ||
| + | ! pSB3K3 (3) | ||
| + | | 0.010 || 0.008 || 1.25 || 30 || 10 || 0.30 | ||
| + | |} | ||
| + | * Since it is impossible to do a successful BioBrick assembly with this small amount of concentraiton we combined all 3 vials of pSB3K3 to create a vial containing 100uL of DNA, and the Measurements were retaken | ||
| + | {| cellpadding="5" border="1" padding="5" | ||
| + | |+ | ||
| + | |- | ||
| + | ! Part No.!! A260 (nm) !! A280 (nm) !! A260/A280 !! Volume (µL) !! Conc. (µg/mL) !!Amount (µg) | ||
| + | |- | ||
| + | ! pSB3K3 (All) | ||
| + | | 0.010 || 0.010 || 1.0 || 97 || 20 || 1.94 | ||
| + | |} | ||
[[User:Trentay|Trentay]] 21:29, 7 July 2009 (UTC) | [[User:Trentay|Trentay]] 21:29, 7 July 2009 (UTC) | ||
Revision as of 20:28, 5 August 2009
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Contents |
Tuesday 7 July 2009
Results
- Everyone is still waiting on electrotransformation cuvettes to proceed with projects
Afton and Maria
- pSB3K3 backbone did not show up on the gel. Only the ladder is visible
Trentay 23:23, 7 July 2009 (UTC)
Tasks
- Continue research and writing paper drafts
Afton and Maria
- Take Spectrometer measurements to get DNA amounts of pSB3K3
- Digestion of pSB3K3
- Work on Excel record of stocks
- Run Electrophoresis test on digestion
Trentay 23:24, 7 July 2009 (UTC)
Craig and Clay
- Since the Eidenburg team was not able to detect the presence of limonene, after transformation, we will see if we are able to use more sensitive equipment to detect its presence. The synthesized sequence for the LIM1 gene will also be studied to see if a more optimized sequence exists for E. coli. It must be noted that the Eidenburg team also deleted a target sequence at the beginning of the LIM1 gene which may have proven to be detrimental to its expression. Furthermore, the pathway that the LIM1 gene competes with may be critical to cell survival. These possible reasons will be more carefully deconstructed.
Wetlab
Afton and Maria
- Digestion
- Agarose Gel preparation/Electrophoresis test
- Spectrometry of Miniprepped DNA
| Part No. | A260 (nm) | A280 (nm) | A260/A280 | Volume (µL) | Conc.(µg/mL) | Amount (µg) |
|---|---|---|---|---|---|---|
| pSB3K3 (1) | 0.011 | 0.010 | 1.10 | 35 | 11 | 0.385 |
| pSB3K3 (2) | 0.010 | 0.008 | 1.25 | 35 | 10 | 0.35 |
| pSB3K3 (3) | 0.010 | 0.008 | 1.25 | 30 | 10 | 0.30 |
- Since it is impossible to do a successful BioBrick assembly with this small amount of concentraiton we combined all 3 vials of pSB3K3 to create a vial containing 100uL of DNA, and the Measurements were retaken
| Part No. | A260 (nm) | A280 (nm) | A260/A280 | Volume (µL) | Conc. (µg/mL) | Amount (µg) |
|---|---|---|---|---|---|---|
| pSB3K3 (All) | 0.010 | 0.010 | 1.0 | 97 | 20 | 1.94 |
Trentay 21:29, 7 July 2009 (UTC)
