From 2009.igem.org
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| + | 2. Restriction digest of R0040, B0015, and J13002 |
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| + | To verify if B0015, R0040, and J13002 are present within the plasmid/cell, restriction digest was performed using NotI enzyme. |
| + | [[Image:Calgary_2009.06.16.B0015_R0040_J13002.plates.png|700px]] |
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| + | The band in R0040 C2 #1 is the only one that did not match the expected size of each. Only one colony is needed to move on. |
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Revision as of 22:25, 5 August 2009
University of Calgary
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CAROL
Descriptive Title of What You’re Doing
WIKI CODING HERE
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CHINMOYEE
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EMILY
Descriptive Title of What You're Doing
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FAHD
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IMAN
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JAMIE
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JEREMY
Descriptive Title of What You're Doing
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KATIE
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KEVIN
1. Plasmid Isolation of R0040, B0015, and J13002
R0040 (promoter), B0015 (terminator), and J13002 (RBS+Promoter) were isolated in order to verify the presence of them with Restriction digest. These parts are needed for general construction of our circuits.
2. Restriction digest of R0040, B0015, and J13002
To verify if B0015, R0040, and J13002 are present within the plasmid/cell, restriction digest was performed using NotI enzyme.
The band in R0040 C2 #1 is the only one that did not match the expected size of each. Only one colony is needed to move on.
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<a name="Mandy"></a>
Descriptive Title of What You're Doing
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PATRICK
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PRIMA
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STEFAN
Descriptive Title of What You're Doing
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VICKI
Descriptive Title of What You're Doing
WIKI CODING HERE
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