Virginia Commonwealth/6 August 2009
From 2009.igem.org
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-[[User:Bussingkm|Bussingkm]] 00:21, 7 August 2009 (UTC) | -[[User:Bussingkm|Bussingkm]] 00:21, 7 August 2009 (UTC) |
Revision as of 00:25, 7 August 2009
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Contents |
Thursday 6 August 2009
Results
Kevin
- Everything that I digested, ligated, and transformed yesterday grew over night. However, I used the wrong plasmid backbone. Both my reporter and backbone insert reported RFP. Will be growing same backbone containing the death gene (BBa_P1010). We will use E. coli strain Db3.1 to grow plasmid. The DNA will be minipreped Friday and digested and ligated on Monday.
-Bussingkm 00:21, 7 August 2009 (UTC)
Tasks
Kevin
- I need to finish writing a progress report for my fellowship. Hopefully the flow cytometer will be fixed soon so that we can finally get some results. Confirmed that whenever we have florescing bacteria ready we can proceed with RT PCR.
-Bussingkm 00:21, 7 August 2009 (UTC)
Wetlab
- Picked colonies for several new plasmids that came in today. The plasmids contained parts:
- BBa_I313600
- BBa_K118024
- BBa_K118025
- BBa_I742111
All parts came on the high copy, ampicillin resistant backbone pSB1A2
-Bussingkm 00:21, 7 August 2009 (UTC)