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Template:Team:KULeuven/7 August 2009/VanillinReceptor
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Didierborowski (Talk | contribs)
(New page: * primers for mutagenesis have arrived * We used the DNA parts from the second PCR to insert them in to a TOPO-vector. The second PCR showed more results so that is why we used this frage...)
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(New page: * primers for mutagenesis have arrived * We used the DNA parts from the second PCR to insert them in to a TOPO-vector. The second PCR showed more results so that is why we used this frage...)
Newer edit →
Revision as of 10:30, 7 August 2009
- primers for mutagenesis have arrived
- We used the DNA parts from the second PCR to insert them in to a TOPO-vector. The second PCR showed more results so that is why we used this fragements.
- LBmedium was made with X-gal to select cells afterwards.
- Plasmids were inserted in competent cells by heatshock and those cells were plated out on the X-gal medium.
