"
Team:Aberdeen Scotland/notebook/lacilatch
From 2009.igem.org
(→Week 4 29/06/09 - 03/07/09) |
|||
| Line 78: | Line 78: | ||
*Miniprep of C0051, R0062, J37033, I732100. | *Miniprep of C0051, R0062, J37033, I732100. | ||
| + | *End of week meeting | ||
==Week 3: Edinburgh iGEM meeting and BioBrick Rescuing 22/06/09 - 26/06/09== | ==Week 3: Edinburgh iGEM meeting and BioBrick Rescuing 22/06/09 - 26/06/09== | ||
| Line 119: | Line 120: | ||
*Restriction Digest of E0840 | *Restriction Digest of E0840 | ||
*Gel Electrophoresis of E0840 | *Gel Electrophoresis of E0840 | ||
| + | *End of week meeting | ||
| + | |||
==Week 4: 29/06/09 - 03/07/09== | ==Week 4: 29/06/09 - 03/07/09== | ||
| Line 161: | Line 164: | ||
*Miniprep PSB3AT3, PSB3AC3 | *Miniprep PSB3AT3, PSB3AC3 | ||
| - | * | + | *End of week meeting |
| + | |||
| + | |||
==Week 5 05/07/09 - 10/07/09== | ==Week 5 05/07/09 - 10/07/09== | ||
Revision as of 10:20, 10 August 2009
University of Aberdeen - Pico Plumber
LacI-Latch Notebook
Week 1: Research and Planning 08/06/09 - 12/06/09
Day 1 Monday (08/06/09)
- Researching the Registry for Biobricks
- Identified K182022, K112808, R0062, I712074, I14015 for further disccusion.
Day 2 Tuesday (09/06/09)
- Researching the Registry for Plasmids.
- Identified pSB3T5, pSB3C5, pSB1AC3, pSB1AT3 for further discusion.
Day 3 Wednesday (10/06/09)
- Literature search for Parameters of T7 promoter and the Holin system.
- Conlusion: T7 feedback mechanism of Wiring diagram 1 is unliekly to be functional without further regulations.
- Identified the novagen plasmids pLysS and pLysE as possible solutions.
- Researching the Registry for LacI Repressor and T7 Polymerase
- Identified K145014, K145001, I732100 for further discusion
Day 4 Thursday (11/06/09)
- Parameter search for the Modeling effort.
Day 5 Friday (12/06/09)
- End of week meeting
- Discussion and planning of following week
Week 2: BioBrick Rescue 15/06/09 - 19/06/09
Day 1 Monday (15/06/09)
- Prepared LB medium for Lab
- Planning of LacI-Latch
- Inoculated J23100, K145001, B0034 for Miniprep the following day
Day 2 Tuesday (16/06/09)
- Miniprep of J23100, K145001, B0034
- Restriction Digest
Day 3 Wednesday (17/06/09)
- Lambda Digest with HindIII for use as a Ladder
- Gel Electrophoresis of B0034, K145001, J23100, I732100.
Day 4 Thursday (18/06/09)
- Gel Electrophoresis of C0051, R0062, C0040, J37033, K145001, I732100.
- Innoculation of C0051, R0062, J37033, I732100 or miniprep.
Day 5 Friday (19/06/09)
- Miniprep of C0051, R0062, J37033, I732100.
- End of week meeting
Week 3: Edinburgh iGEM meeting and BioBrick Rescuing 22/06/09 - 26/06/09
Day 1 Monday (22/06/09)
- Rescued BBa_1732820, S03518, B0030
Day 2 Tuesday (23/06/09)
Risat:
- Research for Plasmids and J series Promoters
- Rescued and Transformed Psb4k4 and Psb3K5.
- Restriction Digest of B0015, R0040, E0840, R0011, K093005, R0051, K081008, J37033, R0062,
Daniel:
- Edinburgh Igem Meeting
Day 3 Wednesday (24/06/09)
Risat:
- Gel Electrophoresis of B0015, R0040, E0840, R0011, K093005, R0051, K081008, J37033, R0062,
Daniel:
- Edinburgh Igem Meeting
Day 4 Thursday (25/06/09)
Risat:
- Gel Electrophoresis of K112808, K112022, I732100
Daniel:
- Edinburgh Igem Meeting
Day 5 Friday (26/06/09)
- Restriction Digest of E0840
- Gel Electrophoresis of E0840
- End of week meeting
Week 4: 29/06/09 - 03/07/09
Day 1 Monday
1. Run Gel of E0840 ( Testing of digests) (9-11) i) Add Dye ii) Setting up and pouring the gels 2. Grow for miniprep for I732820 (LacI) R0051 (CI operator) S03518 ( TetR) K112808 ( Holin) PSB3k3, PSB3T5, i) Put colonies into plate and test tube for incubation. ii) (reason for this is we realised that we need a lot more DNA for ligations.) 3. Rescue I13600 and I13601
Day 2 Tuesday
1. Miniprep for I732820 (LacI) R0051 (CI operator) S03518 ( TetR) K112808 ( Holin) PSB3k3, PSB3T5 i) Miniprep ii) Planning for digest • R0051, S03518, R0040, PSB3k3, I732820. iii) Set up digestions iv) Get digestions out and run gel v) Interpret results 2. Transformation’s of I13600, I13601 3. Grow for Miniprep PSB4K5, PSB3K3
Day 3 Wednesday
1. Miniprep PSB4K5, PSB3K3 2. Restriction Digest of PSB4k5, PSB3K3, S03518, R0040 3. Gel electrophoresis 4. Grow for Miniprep PSB1AT3, PSB1AC3
Day 4 Thursday
1. Transform I13600 and I13601 2. Run Gel of R0040 3. Incoculate for Miniprep, PSB3AT3, PSB3AC3.
Day 5 Friday
- Miniprep PSB3AT3, PSB3AC3
- End of week meeting
Week 5 05/07/09 - 10/07/09
Week 6 13/07/09 - 16/07/09
Week 7 20/07/09 - 24/07/09
Week 8 27/07/09 - 31/07/09
Week 9 03/08/09 - 07/08/09
Week 10 10/08/09 - 14/08/09
Week 11 17/08/09 - 21/08/09
 Back
|
Continue 
|
