Team:Aberdeen Scotland/notebook/lacilatch

From 2009.igem.org

(Difference between revisions)
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==Week 1: Research and Planning 08/06/09 - 12/06/09==
==Week 1: Research and Planning 08/06/09 - 12/06/09==
-
 
+
<br><br>
===Day 1 Monday (08/06/09)===
===Day 1 Monday (08/06/09)===
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**Identified pSB3T5, pSB3C5, pSB1AC3, pSB1AT3 for further discusion.
**Identified pSB3T5, pSB3C5, pSB1AC3, pSB1AT3 for further discusion.
-
 
+
<br><br>
-
 
+
===Day 3 Wednesday (10/06/09)===
===Day 3 Wednesday (10/06/09)===
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** Identified the novagen plasmids pLysS and pLysE as possible solutions.
** Identified the novagen plasmids pLysS and pLysE as possible solutions.
-
 
+
<br><br>
-
 
+
*Researching the Registry for LacI Repressor and T7 Polymerase
*Researching the Registry for LacI Repressor and T7 Polymerase
** Identified K145014, K145001, I732100 for further discusion
** Identified K145014, K145001, I732100 for further discusion
-
 
+
<br><br>
===Day 4 Thursday (11/06/09)===
===Day 4 Thursday (11/06/09)===
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-
 
+
<br><br>
===Day 5 Friday (12/06/09)===
===Day 5 Friday (12/06/09)===
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*Discussion and planning of following week
*Discussion and planning of following week
 +
<br><br>
==Week 2: BioBrick Rescue  15/06/09 - 19/06/09==
==Week 2: BioBrick Rescue  15/06/09 - 19/06/09==
-
 
+
<br><br>
===Day 1 Monday (15/06/09)===
===Day 1 Monday (15/06/09)===
-
*Prepared LB medium for Lab
+
*Prepared LB medium for Lab<br><br>
-
*Planning of LacI-Latch
+
*Planning of LacI-Latch<br><br>
*Inoculated J23100, K145001, B0034 for Miniprep the following day
*Inoculated J23100, K145001, B0034 for Miniprep the following day
-
 
+
<br><br>
===Day 2 Tuesday (16/06/09)===
===Day 2 Tuesday (16/06/09)===
-
*Miniprep of J23100, K145001, B0034
+
*Miniprep of J23100, K145001, B0034<br><br>
-
*Restriction Digest  
+
*Restriction Digest <br><br>
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===Day 3 Wednesday (17/06/09)===
===Day 3 Wednesday (17/06/09)===
-
* Lambda Digest with HindIII for use as a Ladder
+
* Lambda Digest with HindIII for use as a Ladder<br><br>
-
* Gel Electrophoresis of B0034, K145001, J23100, I732100.
+
* Gel Electrophoresis of B0034, K145001, J23100, I732100.<br><br>
===Day 4 Thursday (18/06/09)===
===Day 4 Thursday (18/06/09)===
-
*Gel Electrophoresis of C0051, R0062, C0040, J37033, K145001, I732100.
+
*Gel Electrophoresis of C0051, R0062, C0040, J37033, K145001, I732100.<br><br>
-
*Innoculation of C0051, R0062, J37033, I732100 or miniprep.
+
*Innoculation of C0051, R0062, J37033, I732100 or miniprep.<br><br>
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===Day 5 Friday (19/06/09)===
===Day 5 Friday (19/06/09)===
-
*Miniprep of C0051, R0062, J37033, I732100.
+
*Miniprep of C0051, R0062, J37033, I732100.<br><br>
-
*End of week meeting
+
*End of week meeting<br><br>
==Week 3: Edinburgh iGEM meeting and BioBrick Rescuing 22/06/09 - 26/06/09==
==Week 3: Edinburgh iGEM meeting and BioBrick Rescuing 22/06/09 - 26/06/09==
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===Day 1 Monday (22/06/09)===
===Day 1 Monday (22/06/09)===
-
*Rescued BBa_1732820, S03518, B0030
+
*Rescued BBa_1732820, S03518, B0030<br><br>
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Risat:
Risat:
-
*Research for Plasmids and J series Promoters
+
*Research for Plasmids and J series Promoters<br><br>
-
*Rescued and Transformed Psb4k4 and Psb3K5.
+
*Rescued and Transformed Psb4k4 and Psb3K5.<br><br>
-
*Restriction Digest of B0015, R0040, E0840, R0011, K093005, R0051, K081008, J37033, R0062,   
+
*Restriction Digest of B0015, R0040, E0840, R0011, K093005, R0051, K081008, J37033, R0062,  <br><br>
Daniel:
Daniel:
-
*Edinburgh Igem Meeting
+
*Edinburgh Igem Meeting<br><br>
===Day 3 Wednesday (24/06/09)===
===Day 3 Wednesday (24/06/09)===
Risat:
Risat:
-
*Gel Electrophoresis of B0015, R0040, E0840, R0011, K093005, R0051, K081008, J37033, R0062,   
+
*Gel Electrophoresis of B0015, R0040, E0840, R0011, K093005, R0051, K081008, J37033, R0062,  <br><br>
Daniel:
Daniel:
-
*Edinburgh Igem Meeting
+
*Edinburgh Igem Meeting<br><br>
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Risat:
Risat:
-
*Gel Electrophoresis of K112808, K112022, I732100
+
*Gel Electrophoresis of K112808, K112022, I732100<br><br>
Daniel:
Daniel:
-
*Edinburgh Igem Meeting
+
*Edinburgh Igem Meeting<br><br>
===Day 5 Friday (26/06/09)===
===Day 5 Friday (26/06/09)===
-
*Restriction Digest of E0840
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*Restriction Digest of E0840<br><br>
-
*Gel Electrophoresis of E0840
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*Gel Electrophoresis of E0840<br><br>
-
*End of week meeting
+
*End of week meeting<br><br>
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===Day 1 Monday===
===Day 1 Monday===
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*Run Gel of E0840 ( Testing of digests)
+
*Run Gel of E0840 ( Testing of digests)<br><br>
*Innoculate for miniprep.  
*Innoculate for miniprep.  
-
** I732820 (LacI,) R0051 (CI operator), S03518 ( TetR), K112808 ( Holin), PSB3k3, PSB3T5
+
** I732820 (LacI,) R0051 (CI operator), S03518 ( TetR), K112808 ( Holin), PSB3k3, PSB3T5.<br><br>
*Rescue I13600 and I13601
*Rescue I13600 and I13601
-
** Required for testing of constructs
+
** Required for testing of constructs<br><br>
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*Miniprep  
*Miniprep  
-
**I732820 (LacI) R0051 (CI operator)  S03518 ( TetR) K112808 ( Holin)  PSB3k3, PSB3T5
+
**I732820 (LacI) R0051 (CI operator)  S03518 ( TetR) K112808 ( Holin)  PSB3k3, PSB3T5.<br><br>
*Planning for Restriction digest  
*Planning for Restriction digest  
-
**R0051, S03518, R0040, PSB3k3, I732820.
+
**R0051, S03518, R0040, PSB3k3, I732820.<br><br>
* Restriction Digest
* Restriction Digest
-
**R0051, S03518, R0040, PSB3k3, I732820.
+
**R0051, S03518, R0040, PSB3k3, I732820.<br><br>
*Gel Electrophoresis
*Gel Electrophoresis
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**R0051, S03518, R0040, PSB3k3, I732820.
+
**R0051, S03518, R0040, PSB3k3, I732820.<br><br>
   
   
*Transformation (using XL1-Blue)  
*Transformation (using XL1-Blue)  
**I13600, I13601
**I13600, I13601
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*** Result : Failed (no growth)
+
*** Result : Failed (no growth)<br><br>
*Innoculation for Miniprep  
*Innoculation for Miniprep  
-
**PSB4K5, PSB3K3
+
**PSB4K5, PSB3K3<br><br>
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*Miniprep  
*Miniprep  
-
**PSB4K5, PSB3K3
+
**PSB4K5, PSB3K3<br><br>
*Restriction Digest  
*Restriction Digest  
-
**PSB4k5, PSB3K3, S03518, R0040
+
**PSB4k5, PSB3K3, S03518, R0040<br><br>
*Gel electrophoresis  
*Gel electrophoresis  
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**PSB4K5, PSB3K3, S03518, R0040
+
**PSB4K5, PSB3K3, S03518, R0040<br><br>
*Innoculate for Miniprep  
*Innoculate for Miniprep  
-
**PSB1AT3, PSB1AC3
+
**PSB1AT3, PSB1AC3<br><br>
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*Transformation (using Xl1-Blue)  
*Transformation (using Xl1-Blue)  
**I13600 and I13601  
**I13600 and I13601  
-
*** Result : Worked
+
*** Result : Worked<br><br>
*Gel Electrophoresis  
*Gel Electrophoresis  
-
**R0040
+
**R0040<br><br>
*Incoculate for Miniprep  
*Incoculate for Miniprep  
-
**PSB3AT3, PSB3AC3
+
**PSB3AT3, PSB3AC3<br><br>
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*Miniprep   
*Miniprep   
-
**PSB3AT3, PSB3AC3
+
**PSB3AT3, PSB3AC3<br><br>
-
*End of week meeting
+
*End of week meeting<br><br>
==Week 5 05/07/09 - 10/07/09==
==Week 5 05/07/09 - 10/07/09==

Revision as of 10:34, 10 August 2009

University of Aberdeen iGEM 2009


Contents

LacI-Latch Notebook

Week 1: Research and Planning 08/06/09 - 12/06/09



Day 1 Monday (08/06/09)

  • Researching the Registry for Biobricks
    • Identified K182022, K112808, R0062, I712074, I14015 for further disccusion.



Day 2 Tuesday (09/06/09)

  • Researching the Registry for Plasmids.
    • Identified pSB3T5, pSB3C5, pSB1AC3, pSB1AT3 for further discusion.



Day 3 Wednesday (10/06/09)

  • Literature search for Parameters of T7 promoter and the Holin system.
    • Conlusion: T7 feedback mechanism of Wiring diagram 1 is unliekly to be functional without further regulations.
    • Identified the novagen plasmids pLysS and pLysE as possible solutions.



  • Researching the Registry for LacI Repressor and T7 Polymerase
    • Identified K145014, K145001, I732100 for further discusion




Day 4 Thursday (11/06/09)

  • Parameter search for the Modeling effort.




Day 5 Friday (12/06/09)

  • End of week meeting
  • Discussion and planning of following week



Week 2: BioBrick Rescue 15/06/09 - 19/06/09



Day 1 Monday (15/06/09)

  • Prepared LB medium for Lab

  • Planning of LacI-Latch

  • Inoculated J23100, K145001, B0034 for Miniprep the following day



Day 2 Tuesday (16/06/09)

  • Miniprep of J23100, K145001, B0034

  • Restriction Digest


Day 3 Wednesday (17/06/09)

  • Lambda Digest with HindIII for use as a Ladder

  • Gel Electrophoresis of B0034, K145001, J23100, I732100.


Day 4 Thursday (18/06/09)

  • Gel Electrophoresis of C0051, R0062, C0040, J37033, K145001, I732100.

  • Innoculation of C0051, R0062, J37033, I732100 or miniprep.


Day 5 Friday (19/06/09)

  • Miniprep of C0051, R0062, J37033, I732100.

  • End of week meeting

Week 3: Edinburgh iGEM meeting and BioBrick Rescuing 22/06/09 - 26/06/09

Day 1 Monday (22/06/09)

  • Rescued BBa_1732820, S03518, B0030


Day 2 Tuesday (23/06/09)

Risat:

  • Research for Plasmids and J series Promoters

  • Rescued and Transformed Psb4k4 and Psb3K5.

  • Restriction Digest of B0015, R0040, E0840, R0011, K093005, R0051, K081008, J37033, R0062,

Daniel:

  • Edinburgh Igem Meeting

Day 3 Wednesday (24/06/09)

Risat:

  • Gel Electrophoresis of B0015, R0040, E0840, R0011, K093005, R0051, K081008, J37033, R0062,

Daniel:

  • Edinburgh Igem Meeting


Day 4 Thursday (25/06/09)

Risat:

  • Gel Electrophoresis of K112808, K112022, I732100

Daniel:

  • Edinburgh Igem Meeting


Day 5 Friday (26/06/09)

  • Restriction Digest of E0840

  • Gel Electrophoresis of E0840

  • End of week meeting


Week 4: 29/06/09 - 03/07/09

Day 1 Monday

  • Run Gel of E0840 ( Testing of digests)


  • Innoculate for miniprep.
    • I732820 (LacI,) R0051 (CI operator), S03518 ( TetR), K112808 ( Holin), PSB3k3, PSB3T5.


  • Rescue I13600 and I13601
    • Required for testing of constructs


Day 2 Tuesday

  • Miniprep
    • I732820 (LacI) R0051 (CI operator) S03518 ( TetR) K112808 ( Holin) PSB3k3, PSB3T5.

  • Planning for Restriction digest
    • R0051, S03518, R0040, PSB3k3, I732820.

  • Restriction Digest
    • R0051, S03518, R0040, PSB3k3, I732820.


  • Gel Electrophoresis
    • R0051, S03518, R0040, PSB3k3, I732820.


  • Transformation (using XL1-Blue)
    • I13600, I13601
      • Result : Failed (no growth)

  • Innoculation for Miniprep
    • PSB4K5, PSB3K3


Day 3 Wednesday

  • Miniprep
    • PSB4K5, PSB3K3


  • Restriction Digest
    • PSB4k5, PSB3K3, S03518, R0040


  • Gel electrophoresis
    • PSB4K5, PSB3K3, S03518, R0040

  • Innoculate for Miniprep
    • PSB1AT3, PSB1AC3


Day 4 Thursday

  • Transformation (using Xl1-Blue)
    • I13600 and I13601
      • Result : Worked

  • Gel Electrophoresis
    • R0040

  • Incoculate for Miniprep
    • PSB3AT3, PSB3AC3


Day 5 Friday

  • Miniprep
    • PSB3AT3, PSB3AC3


  • End of week meeting

Week 5 05/07/09 - 10/07/09

Week 6 13/07/09 - 16/07/09

Week 7 20/07/09 - 24/07/09

Week 8 27/07/09 - 31/07/09

Week 9 03/08/09 - 07/08/09

Week 10 10/08/09 - 14/08/09

Week 11 17/08/09 - 21/08/09

  Back Continue  

 

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