Team:KULeuven/Freezer

From 2009.igem.org

(Difference between revisions)
(→vanillin receptor)
(→Blue light receptor)
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| 2172 || / || reverse primer for {{kulpart|BBa_K238000}} || || || 100μM || july 24
| 2172 || / || reverse primer for {{kulpart|BBa_K238000}} || || || 100μM || july 24
|-
|-
 +
| 2260 || / || forward primer for {{kulpart|BBa_K238013}} || || 45µl || 20μM ||aug 10
 +
|-
 +
| 2261 || / || reverse primer for {{kulpart|BBa_K238013}} || || 45µl || 20μM || aug 10
 +
|-
 +
| 2260 || / || forward primer for {{kulpart|BBa_K238013}} || || || 100μM || aug 10
 +
|-
 +
| 2261 || / || reverse primer for {{kulpart|BBa_K238013}} || || || 100μM || aug 10
 +
| {{kulpart|BBa_E0240}}||pSB1A2||GFP + rbs + terminator||purified||||||july 29
| {{kulpart|BBa_E0240}}||pSB1A2||GFP + rbs + terminator||purified||||||july 29
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|-
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|-
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| {{kulpart|BBa_K238000}}||/||BLR promoter region||4 microcentrifuge tubes||||||july 30
| {{kulpart|BBa_K238000}}||/||BLR promoter region||4 microcentrifuge tubes||||||july 30
 +
 +
| {{kulpart|BBa_K238013}}||/||BLR promoter region||1 microcentrifuge tube||||||aug 10
|}
|}

Revision as of 13:16, 10 August 2009

Contents

Blue light receptor

part plasmid description status μl left concentration date
MC4100 / strain of E.coli july 24
2171 / forward primer for 90µl 20μM july 24
2172 / reverse primer for 90µl 20μM july 24
2171 / forward primer for 100μM july 24
2172 / reverse primer for 100μM july 24
2260 / forward primer for 45µl 20μM aug 10
2261 / reverse primer for 45µl 20μM aug 10
2260 / forward primer for 100μM aug 10
2261 / reverse primer for 100μM aug 10 pSB1A2GFP + rbs + terminatorpurifiedjuly 29
pSB1A2GFP + rbs + terminatorrestricted with EcoRI and XbaIjuly 29
/BLR promoter regionrestricted with EcoRIjuly 29
/BLR promoter region4 microcentrifuge tubesjuly 30 /BLR promoter region1 microcentrifuge tubeaug 10

Key/antikey/lock

part plasmid description status μl left concentration date
/GFP with LVA tagpurifiedjuly 28
/tetR promoter purifiedjuly 28

vanillin synthesis

part plasmid description status μl left concentration date
pSB1A2ech + rbspurified plasmid108,8 ng/µlAugust 4
pSB1A2fcs + rbspurified plasmid113,9 ng/µlAugust 4
pSB1A2Sam5 + rbsreceived from EdinburghAugust 3
pSB1A2Sam5 + rbs, PstI site removedpurified plasmid101,3 ng/µlAugust 5
pSB1A2Sam8 + rbspurified plasmid95,7 ng/µlAugust 5
pSB1A2Sam8purified plasmid81,5 ng/µlAugust 5
pSB1A2COMTpurified plasmid122,9 ng/µlAugust 5
pSB1A2COMT + rbsplasmid from registryAugust 6
pSB1A2ech + rbspurified digest16,9 ng/µlAugust 6
fcs + rbspurified digest16,6 ng/µlAugust 6
pSB1A2sam5 + rbspurified digest3,1 ng/µlAugust 7
sam8 + rbspurified digest3,4 ng/µl August 7

vanillin receptor

part plasmid description status μl left concentration date

|- |||pSB1A2||ech + rbs||purified plasmid||||108,8 ng/µl||August 4 |- |||pSB1A2||fcs + rbs||purified plasmid||||113,9 ng/µl||August 4 |- |||pSB1A2||Sam5 + rbs||received from Edinburgh||||||August 3 |- |||pSB1A2||Sam5 + rbs, PstI site removed||purified plasmid||||101,3 ng/µl||August 5

miscellaneous

part plasmid description status μl left concentration date
dNTP mixATP, GTP, TTP, CTP9410μMJuly 24
pSB1A2medium constitutieve promoterJuly 23
ampicilline
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