Virginia Commonwealth/16 July 2009
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===Results=== | ===Results=== | ||
* Results of Retransformation with pSB4C5 backbone in NEB 10 beta two days after transformation | * Results of Retransformation with pSB4C5 backbone in NEB 10 beta two days after transformation | ||
- | {| cellpadding=" | + | {| cellpadding="5" border="1" padding="5" |
|+ | |+ | ||
|- | |- | ||
- | ! Promoter!! Reporter !! | + | ! Promoter!! Reporter !! Plate !! Growth Observation |
|- | |- | ||
! J23101 | ! J23101 | ||
- | | E0240 || Cm || no growth | + | | E0240 || Cm || no growth |
|- | |- | ||
! J23101 | ! J23101 | ||
- | | J06702 || Cm || 21 colonies | + | | J06702 || Cm || 21 colonies |
|- | |- | ||
! J23102 | ! J23102 | ||
- | | E0240 || Cm || 3 colonies | + | | E0240 || Cm || 3 colonies |
|- | |- | ||
! J23102 | ! J23102 | ||
- | | J06702 || Cm || 8 colonies | + | | J06702 || Cm || 8 colonies |
|- | |- | ||
! J23103 | ! J23103 | ||
- | | E0240 || Cm || 2 colonies | + | | E0240 || Cm || 2 colonies |
|- | |- | ||
! J23103 | ! J23103 | ||
- | | J06702 || Cm || 23 colonies | + | | J06702 || Cm || 23 colonies |
|- | |- | ||
! J23104 | ! J23104 | ||
- | | E0240 || Cm || 9 colonies | + | | E0240 || Cm || 9 colonies |
|- | |- | ||
! J23104 | ! J23104 | ||
- | | J06702 || Cm || 12 colonies | + | | J06702 || Cm || 12 colonies |
|- | |- | ||
! J23105 | ! J23105 | ||
- | | E0240 || Cm || no growth | + | | E0240 || Cm || no growth |
|- | |- | ||
! J23105 | ! J23105 | ||
- | | J06702 || Cm || 14 colonies | + | | J06702 || Cm || 14 colonies |
|- | |- | ||
! J23107 | ! J23107 | ||
- | | E0240 || Cm || 3 colonies | + | | E0240 || Cm || 3 colonies |
|- | |- | ||
! J23107 | ! J23107 | ||
- | | J06702 || Cm || 3 colonies | + | | J06702 || Cm || 3 colonies |
|} | |} | ||
Revision as of 16:44, 10 August 2009
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Contents |
Thursday 16 July 2009
Results
- Results of Retransformation with pSB4C5 backbone in NEB 10 beta two days after transformation
Promoter | Reporter | Plate | Growth Observation |
---|---|---|---|
J23101 | E0240 | Cm | no growth |
J23101 | J06702 | Cm | 21 colonies |
J23102 | E0240 | Cm | 3 colonies |
J23102 | J06702 | Cm | 8 colonies |
J23103 | E0240 | Cm | 2 colonies |
J23103 | J06702 | Cm | 23 colonies |
J23104 | E0240 | Cm | 9 colonies |
J23104 | J06702 | Cm | 12 colonies |
J23105 | E0240 | Cm | no growth |
J23105 | J06702 | Cm | 14 colonies |
J23107 | E0240 | Cm | 3 colonies |
J23107 | J06702 | Cm | 3 colonies |
- No growth was seen in 5mL culture taken from NEB 10 beta cell freezer stock
- Vial may have thawed out too much after cells were taken out for the electrocompetency procedure
Trentay 14:31, 16 July 2009 (UTC)
Craig and Clay
- No growth was seen on either the kanamycin or chloramphenicol plates.
- The sequence for the LIM1 gene was verified to be optimized for E. coli.
Tasks
Maria and Afton
- Digest: E0240,J06702, pSB4C5, 110, 113, 116, 0011, 0040
- Ligate promoters with backbone pSB4C5 and reporters E0240, J06702
- Run an electrophoresis test on promoters and reporter Eo240
- Pick colonies of promoters from transformation 100-107 (excluding 106)
- Transformation of NEB 10 beta to make sure new stock is electrocompetent
Trentay 20:41, 16 July 2009 (UTC)
Craig and Clay
- The registry will be contacted to verify the correct resistence for the limonene synthesis related parts. New DNA will also be requested.
Wetlab
Maria and Afton
- Digest: E0240,J06702, pSB4C5, 110, 113, 116, 0011, 0040
- Ligate promoters with backbone pSB4C5 and reporters E0240, J06702
- Run an electrophoresis test on promoters and reporter Eo240
Trentay 20:41, 16 July 2009 (UTC)