Template:Team:KULeuven/12 August 2009/VanillinReceptor

From 2009.igem.org

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* B,G and R were stored for further procedure. A failed again because there was no growth visible. This second time there was no problem with the competent cells because we used a commercial product and not self made. We assumed there was a problem with adding the polyA tales to the PCR product because we did a new gelelectroforesis and there were no errors with the PCR product.
* B,G and R were stored for further procedure. A failed again because there was no growth visible. This second time there was no problem with the competent cells because we used a commercial product and not self made. We assumed there was a problem with adding the polyA tales to the PCR product because we did a new gelelectroforesis and there were no errors with the PCR product.
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*We did a new PCR on the bacteria with the inserted plasmids for B,G and R to check if the gene certainly correct was inserted.
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*We did a new PCR on the bacteria with the inserted plasmids for B,G and R to check if the gene certainly was inserted correct.

Revision as of 11:57, 12 August 2009

  • B,G and R were stored for further procedure. A failed again because there was no growth visible. This second time there was no problem with the competent cells because we used a commercial product and not self made. We assumed there was a problem with adding the polyA tales to the PCR product because we did a new gelelectroforesis and there were no errors with the PCR product.
  • We did a new PCR on the bacteria with the inserted plasmids for B,G and R to check if the gene certainly was inserted correct.
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