Team:KULeuven/Freezer

From 2009.igem.org

(Difference between revisions)
(→vanillin receptor)
(→vanillin synthesis)
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! class='top' | date
! class='top' | date
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|{{kulpart|BBa_I742113}}||pSB1A2||ech + rbs||purified plasmid (C2)||||108,8 ng/µl||August 4
+
|{{kulpart|BBa_I742113}}||pSB1A2||ech + rbs||purified plasmid (oranje)||||108,8 ng/µl||August 4
|-
|-
-
|{{kulpart|BBa_I742115}}||pSB1A2||fcs + rbs||purified plasmid (C1)||||113,9 ng/µl||August 4
+
|{{kulpart|BBa_I742115}}||pSB1A2||fcs + rbs||purified plasmid (oranje)||||113,9 ng/µl||August 4
|-
|-
|{{kulpart|BBa_I742105}}||pSB1A2||Sam5 + rbs||received from Edinburgh||||||August 3
|{{kulpart|BBa_I742105}}||pSB1A2||Sam5 + rbs||received from Edinburgh||||||August 3
|-
|-
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|{{kulpart|BBa_K238007}}||pSB1A2||Sam5 + rbs, PstI site removed||purified plasmid (A2)||||101,3 ng/µl||August 5
+
|{{kulpart|BBa_K238007}}||pSB1A2||Sam5 + rbs, PstI site removed||purified plasmid (oranje)||||101,3 ng/µl||August 5
|-
|-
-
|{{kulpart|BBa_I742106}}||pSB1A2||Sam8 + rbs||purified plasmid (A1)||||95,7 ng/µl||August 5
+
|{{kulpart|BBa_I742106}}||pSB1A2||Sam8 + rbs||purified plasmid (oranje)||||95,7 ng/µl||August 5
|-
|-
-
|{{kulpart|BBa_I742142}}||pSB1A2||Sam8 (b)||purified plasmid||||81,5 ng/µl||August 5
+
|{{kulpart|BBa_I742142}}||pSB1A2||Sam8 (b)||purified plasmid (oranje)||||81,5 ng/µl||August 5
|-
|-
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|{{kulpart|BBa_I742107}}||pSB1A2||COMT||purified plasmid (B2)||||122,9 ng/µl||August 5
+
|{{kulpart|BBa_I742107}}||pSB1A2||COMT||purified plasmid (oranje)||||122,9 ng/µl||August 5
|-
|-
|{{kulpart|BBa_I742109}}||pSB1A2||COMT + rbs||plasmid from registry||0 µl||||August 6
|{{kulpart|BBa_I742109}}||pSB1A2||COMT + rbs||plasmid from registry||0 µl||||August 6
|-
|-
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|||pSB1A2||ech + rbs||purified digest||||16,9 ng/µl||August 6
+
|||pSB1A2||ech + rbs||purified digest (C2)||||16,9 ng/µl||August 6
|-
|-
-
|||||fcs + rbs||purified digest||||16,6 ng/µl||August 6
+
|||||fcs + rbs||purified digest (C1)||||16,6 ng/µl||August 6
|-
|-
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|||pSB1A2||sam5 + rbs||purified digest||||3,1 ng/µl||August 7
+
|||pSB1A2||sam5 + rbs||purified digest (A2)||||3,1 ng/µl||August 7
|-
|-
-
|||||sam8 + rbs||purified digest||||3,4 ng/µl ||August 7
+
|||||sam8 + rbs||purified digest (A1)||||3,4 ng/µl ||August 7
|-
|-
|{{kulpart|BBa_I742115}}||pSB1A2||fcs + rbs||purified plasmid (fcs pl.)||41 µl||57,2 ng/µl||August 12
|{{kulpart|BBa_I742115}}||pSB1A2||fcs + rbs||purified plasmid (fcs pl.)||41 µl||57,2 ng/µl||August 12

Revision as of 12:30, 12 August 2009

Contents

Blue light receptor

part plasmid description status μl left concentration date
MC4100 / strain of E.coli july 24
2171 / forward primer for 90µl 20μM july 24
2172 / reverse primer for 90µl 20μM july 24
2171 / forward primer for 100μM july 24
2172 / reverse primer for 100μM july 24
2260 / forward primer for 45µl 20μM aug 10
2261 / reverse primer for 45µl 20μM aug 10
2260 / forward primer for 100μM aug 10
2261 / reverse primer for 100μM aug 10
pSB1A2GFP + rbs + terminatorrestricted with EcoRI and XbaIjuly 29
/BLR promoter region (1:200 adn 1:500)restricted with EcoRIjuly 29
/PCR BLR promoter region (360bp)4 microcentrifuge tubesjuly 30
Template:IGempart pSB3K3 kanamycine plasmide backbone taken from plates 13μl aug 11
Template:IGempart/constitutive promotortaken from plates 13μl aug 11
Template:IGempart/Exact blue light promotor cut with EcoRI and SpeI 26μl 27 ng/μl /aug 12

Key/antikey/lock

part plasmid description status μl left concentration date
/GFP with LVA tagpurifiedjuly 28
/tetR promoter purifiedjuly 28

vanillin synthesis

part plasmid description status μl left concentration date
pSB1A2ech + rbspurified plasmid (oranje)108,8 ng/µlAugust 4
pSB1A2fcs + rbspurified plasmid (oranje)113,9 ng/µlAugust 4
pSB1A2Sam5 + rbsreceived from EdinburghAugust 3
pSB1A2Sam5 + rbs, PstI site removedpurified plasmid (oranje)101,3 ng/µlAugust 5
pSB1A2Sam8 + rbspurified plasmid (oranje)95,7 ng/µlAugust 5
pSB1A2Sam8 (b)purified plasmid (oranje)81,5 ng/µlAugust 5
pSB1A2COMTpurified plasmid (oranje)122,9 ng/µlAugust 5
pSB1A2COMT + rbsplasmid from registry0 µlAugust 6
pSB1A2ech + rbspurified digest (C2)16,9 ng/µlAugust 6
fcs + rbspurified digest (C1)16,6 ng/µlAugust 6
pSB1A2sam5 + rbspurified digest (A2)3,1 ng/µlAugust 7
sam8 + rbspurified digest (A1)3,4 ng/µl August 7
pSB1A2fcs + rbspurified plasmid (fcs pl.)41 µl57,2 ng/µlAugust 12
pSB1A2Sam5 + rbspurified plasmid (sam5 pl.)43 µl76,0 ng/µlAugust 12
pSB1A2Sam8 + rbspurified plasmid (sam8 pl.)17 µl45,2 ng/µlAugust 12

vanillin receptor

part plasmid description status μl left concentration date
/ forward primer for 20μM August 3
/ reverse primer for 20μM August 3
/ forward primer for 100μM August 3
/ reverse primer for 100μM August 3
/ forward primer for 20μM August 3
/ reverse primer for 20μM August 3
/ forward primer for 100μM August 3
/ reverse primer for 100μM August 3
/ forward primer for 20μM August 3
/ reverse primer for 20μM August 3
/ forward primer for 100μM August 3
/ reverse primer for 100μM August 3
/ forward primer for 20μM August 3
/ reverse primer for 20μM August 3
/ forward primer for 100μM August 3
/ reverse primer for 100μM August 3

miscellaneous

part plasmid description status μl left concentration date
dNTP mixATP, GTP, TTP, CTP9410μMaug 10
pSB1A2medium constitutieve promoterjuly 23
ampicilline
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