Team:Virginia/12 August 2009
From 2009.igem.org
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*Ligated and transformed A09-12 (pSB3K3 + ArsR + E0240) | *Ligated and transformed A09-12 (pSB3K3 + ArsR + E0240) | ||
*New PCR to add HA to PCS. Not using a separated cycle instead running 29 cycles at 65C annealing temp | *New PCR to add HA to PCS. Not using a separated cycle instead running 29 cycles at 65C annealing temp | ||
+ | *Amplifying more product for RFP, GFP and rRNA fragments |
Revision as of 20:19, 12 August 2009
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Overnight Results
- A09-11 had colonies and satellite colonies
Today
- Ligated and transformed A09-12 (pSB3K3 + ArsR + E0240)
- New PCR to add HA to PCS. Not using a separated cycle instead running 29 cycles at 65C annealing temp
- Amplifying more product for RFP, GFP and rRNA fragments