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Virginia Commonwealth/13 August 2009
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===Tasks=== | ===Tasks=== | ||
| - | * 1 | + | * 1 Run PCR reaction [[User:Bussingkm|Bussingkm]] 15:42, 13 August 2009 (UTC) |
* 2 | * 2 | ||
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====Wetlab==== | ====Wetlab==== | ||
| - | * 1 | + | * 1 The purified PCR product from yesterday's reaction did not show up on a 2% agarose gel next to Hyperlader IV. The 100 BP line of the ladder was visible but the product was not. Either the purification was bad or the results ran off the gel. I think there was an error in purification. I will re-do the PCR reaction of F and R primers onto promoter designs 1-9. I will first optimize Mg2+ concentration with D9+F+R. All reagents will be frozen and kept on ICE as much as possible. I will be using the PCR protocol in the protocol section and will be using Taq mixture for the reaction. A gel with each sample from D9 will be run to confirm the reaction. The expected length of D9 is 148 bp. I will use a 100 bp ladder. [[User:Bussingkm|Bussingkm]] 15:42, 13 August 2009 (UTC) |
* 2 | * 2 | ||
Revision as of 15:42, 13 August 2009
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Contents |
Thursday 13 August 2009
Results
- 1
- 2
Tasks
- 1 Run PCR reaction Bussingkm 15:42, 13 August 2009 (UTC)
- 2
Wetlab
- 1 The purified PCR product from yesterday's reaction did not show up on a 2% agarose gel next to Hyperlader IV. The 100 BP line of the ladder was visible but the product was not. Either the purification was bad or the results ran off the gel. I think there was an error in purification. I will re-do the PCR reaction of F and R primers onto promoter designs 1-9. I will first optimize Mg2+ concentration with D9+F+R. All reagents will be frozen and kept on ICE as much as possible. I will be using the PCR protocol in the protocol section and will be using Taq mixture for the reaction. A gel with each sample from D9 will be run to confirm the reaction. The expected length of D9 is 148 bp. I will use a 100 bp ladder. Bussingkm 15:42, 13 August 2009 (UTC)
- 2
