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Team:KULeuven/Freezer
From 2009.igem.org
(Difference between revisions)
(→vanillin receptor) |
(→vanillin receptor) |
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| 2235 || / ||forward primer for {{kulpart|BBa_K238008}} || || || 20μM || August 3 | | 2235 || / ||forward primer for {{kulpart|BBa_K238008}} || || || 20μM || August 3 | ||
|- | |- | ||
| - | | || / || reverse primer for {{kulpart|BBa_K238008}} || || || 20μM || August 3 | + | | 2238 || / || reverse primer for {{kulpart|BBa_K238008}} || || || 20μM || August 3 |
|- | |- | ||
| 2235 || / || forward primer for {{kulpart|BBa_K238008}} || || || 100μM || August 3 | | 2235 || / || forward primer for {{kulpart|BBa_K238008}} || || || 100μM || August 3 | ||
|- | |- | ||
| - | | || / || reverse primer for {{kulpart|BBa_K238008}} || || || 100μM || August 3 | + | | 2238 || / || reverse primer for {{kulpart|BBa_K238008}} || || || 100μM || August 3 |
|- | |- | ||
| - | | || / || forward primer for {{kulpart|BBa_K238009}} || || || 20μM ||August 3 | + | | 2242 || / || forward primer for {{kulpart|BBa_K238009}} || || || 20μM ||August 3 |
|- | |- | ||
| - | | || / || reverse primer for {{kulpart|BBa_K238009}} || || || 20μM || August 3 | + | | 2243 || / || reverse primer for {{kulpart|BBa_K238009}} || || || 20μM || August 3 |
|- | |- | ||
| - | | || / || forward primer for {{kulpart|BBa_K238009}} || || || 100μM || August 3 | + | | 2242 || / || forward primer for {{kulpart|BBa_K238009}} || || || 100μM || August 3 |
|- | |- | ||
| - | | || / || reverse primer for {{kulpart|BBa_K238009}} || || || 100μM || August 3 | + | | 2243 || / || reverse primer for {{kulpart|BBa_K238009}} || || || 100μM || August 3 |
|- | |- | ||
| || / ||forward primer for {{kulpart|BBa_K238010}} || || || 20μM || August 3 | | || / ||forward primer for {{kulpart|BBa_K238010}} || || || 20μM || August 3 | ||
Revision as of 09:41, 18 August 2009
Contents |
Blue light receptor
| part | plasmid | description | status | μl left | concentration | date |
|---|---|---|---|---|---|---|
| MC4100 | / | strain of E.coli | july 24 | |||
| 2171 | / | forward primer for | 90µl | 20μM | july 24 | |
| 2172 | / | reverse primer for | 90µl | 20μM | july 24 | |
| 2171 | / | forward primer for | 100μM | july 24 | ||
| 2172 | / | reverse primer for | 100μM | july 24 | ||
| 2260 | / | forward primer for | 45µl | 20μM | aug 10 | |
| 2261 | / | reverse primer for | 45µl | 20μM | aug 10 | |
| 2260 | / | forward primer for | 100μM | aug 10 | ||
| 2261 | / | reverse primer for | 100μM | aug 10 | ||
| pSB1A2 | GFP + rbs + terminator | restricted with EcoRI and XbaI | july 29 | |||
| / | BLR promoter region (1:200 adn 1:500) | restricted with EcoRI | july 29 | |||
| / | PCR BLR promoter region (360bp) | 4 microcentrifuge tubes | july 30 | |||
| Template:IGempart | pSB3K3 | kanamycine plasmide backbone | taken from plates | 13μl | aug 11 | |
| Template:IGempart | / | constitutive promotor | taken from plates | 13μl | aug 11 | |
| Template:IGempart | / | Exact blue light promotor | cut with EcoRI and SpeI | 26μl | 27 ng/μl | aug 12 |
| Template:IGempart | / | Exact blue light promotor ligated with E0240 in pSB1A2 | T4 DNA ligation | 18μl | aug 13 | |
| Template:IGempart | / | Exact blue light promotor ligated with K145015 in pSB1A2 | T4 DNA ligation | 18μl | aug 14 |
Key/antikey/lock
| part | plasmid | description | status | μl left | concentration | date |
|---|---|---|---|---|---|---|
| / | GFP with LVA tag | purified | july 28 | |||
| / | tetR promoter | purified | july 28 |
vanillin synthesis
| part | plasmid | description | status | μl left | concentration | date |
|---|---|---|---|---|---|---|
| pSB1A2 | ech + rbs | purified plasmid (oranje) | 108,8 ng/µl | August 4 | ||
| pSB1A2 | fcs + rbs | purified plasmid (oranje) | 113,9 ng/µl | August 4 | ||
| pSB1A2 | Sam5 + rbs | received from Edinburgh | August 3 | |||
| pSB1A2 | Sam5 + rbs, PstI site removed | purified plasmid (oranje) | 101,3 ng/µl | August 5 | ||
| pSB1A2 | Sam8 + rbs | purified plasmid (oranje) | 95,7 ng/µl | August 5 | ||
| pSB1A2 | Sam8 (b) | purified plasmid (oranje) | 81,5 ng/µl | August 5 | ||
| pSB1A2 | COMT | purified plasmid (oranje) | 122,9 ng/µl | August 5 | ||
| pSB1A2 | COMT + rbs | plasmid from registry | 0 µl | August 6 | ||
| pSB1A2 | ech + rbs | purified digest (C2) | 16,9 ng/µl | August 6 | ||
| fcs + rbs | purified digest (C1, FCS Rb) | 16,6 ng/µl | August 6 | |||
| pSB1A2 | sam5 + rbs | purified digest (A2) | 3,1 ng/µl | August 7 | ||
| sam8 + rbs | purified digest (A1) | 3,4 ng/µl | August 7 | |||
| pSB1A2 | sam5 + sam8 | ligation (lig. A) | August 11 | |||
| pSB1A2 | ech + fcs | ligation (lig.C) | August 11 | |||
| pSB1A2 | fcs + rbs | purified plasmid (fcs pl.) | 39 µl | 57,2 ng/µl | August 12 | |
| pSB1A2 | Sam5 + rbs | purified plasmid (sam5 pl.) | 41 µl | 76,0 ng/µl | August 12 | |
| pSB1A2 | Sam8 + rbs | purified plasmid (sam8 pl.) | 0 µl | 45,2 ng/µl | August 12 | |
| fcs + rbs | purified digest (F rd.) | 28 µl | 4,2 ng/µl | August 12 | ||
| pSB1A2 | sam5 + rbs | purified digest (5 rd.) | 28 µl | 7,3 ng/µl | August 12 | |
| pSB1A2 | ech + rbs | purified plasmid (ech pl.) | 48 µl | 72,7 ng/µl | August 13 | |
| pSB1A2 | Sam8 + rbs | purified plasmid (sam8 pl.) | 48 µl | 66,2 ng/µl | August 13 | |
| pSB1A2 | sam5 + sam8 | cells with sam5+sam8 (elek. A) | 600 µl | August 13 | ||
| pSB1A2 | ech + fcs | cells with ech+fcs (elek. C) | 600 µl | August 13 | ||
| sam8 + rbs | purified digest (sam8 rb.) | 26 µl | 9,4 ng/µl | August 14 | ||
| pSB1A2 | ech + rbs | purified digest (ech rb.) | 26 µl | 13,6 ng/µl | August 14 | |
| pSB1A2 | sam8 + Sam5 | ligation (SAMS) | 28 µl | August 14 | ||
| pSB1A2 | ech + fcs | ligation (EF) | 28 µl | August 14 |
vanillin receptor
| part | plasmid | description | status | μl left | concentration | date |
|---|---|---|---|---|---|---|
| 2235 | / | forward primer for | 20μM | August 3 | ||
| 2238 | / | reverse primer for | 20μM | August 3 | ||
| 2235 | / | forward primer for | 100μM | August 3 | ||
| 2238 | / | reverse primer for | 100μM | August 3 | ||
| 2242 | / | forward primer for | 20μM | August 3 | ||
| 2243 | / | reverse primer for | 20μM | August 3 | ||
| 2242 | / | forward primer for | 100μM | August 3 | ||
| 2243 | / | reverse primer for | 100μM | August 3 | ||
| / | forward primer for | 20μM | August 3 | |||
| / | reverse primer for | 20μM | August 3 | |||
| / | forward primer for | 100μM | August 3 | |||
| / | reverse primer for | 100μM | August 3 | |||
| / | forward primer for | 20μM | August 3 | |||
| / | reverse primer for | 20μM | August 3 | |||
| / | forward primer for | 100μM | August 3 | |||
| / | reverse primer for | 100μM | August 3 |
miscellaneous
| part | plasmid | description | status | μl left | concentration | date |
|---|---|---|---|---|---|---|
| dNTP mix | ATP, GTP, TTP, CTP | 94 | 10μM | aug 10 | ||
| pSB1A2 | medium constitutieve promoter | july 23 | ||||
| ampicilline |
Categories: Team:KULeuven/BioBricks | Team:KULeuven/BioBricks/BBa K238000 | Team:KULeuven/BioBricks/BBa K238013 | Team:KULeuven/BioBricks/BBa E0240 | Team:KULeuven/BioBricks/BBa K145015 | Team:KULeuven/BioBricks/BBa K145201 | Team:KULeuven/BioBricks/BBa I742113 | Team:KULeuven/BioBricks/BBa I742115 | Team:KULeuven/BioBricks/BBa I742105 | Team:KULeuven/BioBricks/BBa K238007 | Team:KULeuven/BioBricks/BBa I742106 | Team:KULeuven/BioBricks/BBa I742142 | Team:KULeuven/BioBricks/BBa I742107 | Team:KULeuven/BioBricks/BBa I742109 | Team:KULeuven/BioBricks/BBa K238008 | Team:KULeuven/BioBricks/BBa K238009 | Team:KULeuven/BioBricks/BBa K238010 | Team:KULeuven/BioBricks/BBa K238011 | Team:KULeuven/BioBricks/J23110
