From 2009.igem.org
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| - | WIKI CODING HERE
| + | *Unfortunately my plates from yesterday of Vicki's construct in TOP10 (J13002-LuxOD47A-B0015) did not have any colonies. So I restrated the plasmid switch from the restriction digest, digesting both the construct in psB1AK3 and the psB1AC3 vector with XbaI and PstI restriction enzymes. I transformed the ligated product into TOP10 cells and plated on C plates. Hopefully these plates will have colonies tomorrow and I can proceed with a Colony PCR to verify that the construct is in the vector. |
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| | + | *Today we also had a team meeting where we had another Q & A session in preparation for aGEM and iGEM. I answered some general questions as well as some human practices questions. This was once again extremely helpful in identifying areas that I don't entirely understand, especially within some of the various sub-projects. |
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Revision as of 23:35, 18 August 2009
University of Calgary
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CAROL
Descriptive Title of What You’re Doing
WIKI CODING HERE
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CHINMOYEE
Descriptive Title of What You're Doing
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EMILY
Restriction Digest and Q & A Session
- Unfortunately my plates from yesterday of Vicki's construct in TOP10 (J13002-LuxOD47A-B0015) did not have any colonies. So I restrated the plasmid switch from the restriction digest, digesting both the construct in psB1AK3 and the psB1AC3 vector with XbaI and PstI restriction enzymes. I transformed the ligated product into TOP10 cells and plated on C plates. Hopefully these plates will have colonies tomorrow and I can proceed with a Colony PCR to verify that the construct is in the vector.
- Today we also had a team meeting where we had another Q & A session in preparation for aGEM and iGEM. I answered some general questions as well as some human practices questions. This was once again extremely helpful in identifying areas that I don't entirely understand, especially within some of the various sub-projects.
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FAHD
Ethics & Outreach and Q & A
Today I continued to work on the ethics paper that I started yesterday. I also added extra information on the precautionary and the pro-actionary framework. I also made a program plan for the ethics conference that we will hold in Second Life ™. The plan includes information such as how we intend to carry out the conference, who are we going to invite, what type of question we will be asking our guest speakers, etc. .
We also had a Q & A with our team today. The questions were about our iGEM in general, synthetic biology, our project this year and specific questions concerning specific aspects of our project. The Q & A was an excellent exercise to prepare our team for the type of question that we will be asked during the aGEM and iGEM competition.
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IMAN
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JAMIE
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JEREMY
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KATIE
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KEVIN
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MANDY
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PATRICK
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PRIMA
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STEFAN
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VICKI
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