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Uppsala-Sweden/19 August 2009
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(New page: {{Uppsala-Sweden Template}} ==Preparing competent cells== ''Continued since yesterday'' We harvested the seed cultures from yesterday and will later inoculate 300 ml of SOB media with on...)
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(New page: {{Uppsala-Sweden Template}} ==Preparing competent cells== ''Continued since yesterday'' We harvested the seed cultures from yesterday and will later inoculate 300 ml of SOB media with on...)
Newer edit →
Revision as of 11:58, 19 August 2009
Preparing competent cells
Continued since yesterday
We harvested the seed cultures from yesterday and will later inoculate 300 ml of SOB media with one seed culter for harvest tomorrow. We also put three seed stocks in the -80 freezer for future use.
Gel run
A gel run was performed to evaluate one of the PCR runs from yesterday
Setup: Media:Example.jpg
Result: 
Another gel run was started to evaluate the other PCR performed yesterday.......
Tansforming ADH2 Z.mobilis
Continued since yesterday
The results form the "on column" digested ADH" was good with many colonies on the 50µl plate. We picked 7 of them and started a Taq evaluation PCR
