"
Team:Calgary/8 July 2009
From 2009.igem.org
(Difference between revisions)
Emily Hicks (Talk | contribs) |
|||
| Line 211: | Line 211: | ||
<br> | <br> | ||
<div class="heading"> | <div class="heading"> | ||
| - | + | DNA Sequencing and cloning of B0015 | |
</div> | </div> | ||
<br> | <br> | ||
<div class="desc"> | <div class="desc"> | ||
</html> | </html> | ||
| - | + | *Sent J13002-LuxOD47E- C3 down for DNA Sequencing this morning to verify the presence of the J13002 promoter within the construct. We will proceed with construction to try to clone in the B0015 terminator. | |
| + | |||
| + | *Performed a restriction digest with EcoRI, XbaI and SpeI restriction enzymes,folowed by Antarctic Phosphotase of the vector (NEB) and ligation with QuikLigase (Invitrogen). Did two trial, trial 1 where the B0015 terminator was treated as the insert and trial 2 where the J13002-LuxOD47E construct was terated as the insert. Ligated product was then transformed into TOP10 cells and plated on C plates (25μL and 50μL) and left for overnight growth. | ||
| + | |||
| + | *Today I also transformed some of my J13002-LuxOD47E construct into KT1144 cells. Because my cells are currenlty in the psB1AC3 vector, where there are intrinsic terminators, the B0015 terminator may not be necessary in roder for my circuit to work in the KT1144 cells (where we are testing them). After transformation, cells were plated on both C and K plates and left for overnight growth. | ||
| + | |||
<html> | <html> | ||
Revision as of 18:12, 19 August 2009
UNIVERSITY OF CALGARY
|
CAROL
Descriptive Title of What You’re Doing
WIKI CODING HERE
|
|
CHINMOYEE
Adjusting Model frame work
Me , Carol and Kevin adjusted our model frame work . Then the whole modelling team had a good discussion with Thane . Before that I read more journals dealing with parameter estimation and looked for rates . I looked at the parameter fit function in simbiology and tried to use this function for our purpose.
|
|
EMILY
DNA Sequencing and cloning of B0015
|
|
FAHD
Descriptive Title of What You're Doing
WIKI CODING HERE
|
|
IMAN
Descriptive Title of What You're Doing
WIKI CODING HERE
|
|
JAMIE
Descriptive Title of What You're Doing
WIKI CODING HERE
|
|
JEREMY
Descriptive Title of What You're Doing
WIKI CODING HERE
|
|
KATIE
Creation and Modification of Scripts and Notecards
I have completed creating instructional and information note cards for the various activities within the virtual lab on our island, including:
Note cards that are almost finished and will be completed by tomorrow include:
I have also been creating and modifying the scripts for PCR, Gel Electrophoresis, Restriction Digest and have just started Bacterial Transformation. They have been modified to use a more user friendly prompt system (using llDialog function in second life scripting language) so asking avatars for information and having them answer looks cleaner and is more intuitive.
I am hoping to have restriction digest functioning by Friday and will be working on finishing notecards, Restriction Digest and Bacterial Transformation in the upcoming days. I will also be organizing a sequencing station, which may be used for a section of the lab activities.
|
|
KEVIN
Restreak of the glowing cells
A brightest colony from yesterday's transformation were restreaked. These cells have to grow overnight, once again.
Blog update
A weekly blog is being done by all sections of our team, including wetlab and modelling which I am part of. Every wednesday is wetlab blog day, so one was written. The following is the link to my post:
Kevin Loves Rainbows
|
|
MANDY
Descriptive Title of What You're Doing
WIKI CODING HERE
|
|
PATRICK
Descriptive Title of What You're Doing
WIKI CODING HERE
|
|
PRIMA
Descriptive Title of What You're Doing
WIKI CODING HERE
|
|
STEFAN
Descriptive Title of What You're Doing
WIKI CODING HERE
|
|
VICKI
Descriptive Title of What You're Doing
WIKI CODING HERE
|
