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Team:Calgary/19 August 2009
From 2009.igem.org
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| - | + | 1. Plasmid Isolation | |
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| - | + | <i>Pqrr4</i>+B0034+K082003 (C1, old), (C3, new), (C4, new), (C7, new) was isolated today using Qiagen's miniprep kit (qiagen) according to the manifacture's specifications. Then the purity and the concentration of the product was measured using the nanodrop utility. The result was good enough for me to go on. | |
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| + | 2. Restriction digest | ||
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| + | The above products of <i>Pqrr4</i>+B0034+K082003 (C1, old), (C3, new), and (C7, new) were then restriction digested using XbaI and PstI. The following is a picture of the gel: | ||
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| + | [[Image:Calgary_2009.08.19.png|500px]] | ||
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| + | All three bands look approximately the same, so the purist, being (C1,old), was picked to be sent for sequencing. Results will be in by tomorrow. | ||
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| + | 3. Overnight cultures | ||
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| + | The overnight cultures of TOP10 cells with pBluescript, Reporter by itself, Reporter and LuxOD47E, R0040 (constitutive promoter) and GFP, KT1144 cells with GFP cosmid, and KT1144 cells with GFP cosmid and LuxOD47E were grown in order for tomorrow's test with Plate reader. | ||
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Revision as of 22:45, 19 August 2009
UNIVERSITY OF CALGARY
