From 2009.igem.org
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| - | Descriptive of What You're Doing
| + | 1. Plasmid Isolation |
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| - | WIKI CODING HERE
| + | I have isolated the <i>Pqrr4</i>+B0034 plasmids from yesterday's overnight cultures, and the purity and concentration of them were measured using nanodrop. They were pure enough to move on with. |
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| | + | 2. Restriction digest |
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| | + | The isolated <i>Pqrr4</i>+B0034 plasmid was then cut with XbaI and PstI and ran on a 2% gel at 90V in order to verify the presence of the circuit. The following is an image of the gel: |
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| | + | [[Image:Calgary_2009.07.21.Pqrr4+B0034_edited.png|700px]] |
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| | + | The lower bands of the colonies 1, 2, 4, 6, 8, 9, and 10 seem to be above the positive control bands, which is what was expected. Thus it is now ready for sequencing. |
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Revision as of 20:09, 20 August 2009
University of Calgary
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CAROL
Making Competent Cells
- Prepared 500mL LB broth for making competent cells. Followed procedure that is outlined in the protocol page. Unfortunately, due to time constraints, we had to re-make the overnight cultures.
- Prepared two 5 mL overnight cultures (LB broth) of Top 10 cells and XL Gold Ultracompetent cells.
- Helped the bioinformatics department (Bachelor of Health Sciences) take apart computers in the morning.
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CHINMOYEE
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EMILY
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FAHD
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IMAN
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JAMIE
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JEREMY
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KATIE
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KEVIN
1. Plasmid Isolation
I have isolated the Pqrr4+B0034 plasmids from yesterday's overnight cultures, and the purity and concentration of them were measured using nanodrop. They were pure enough to move on with.
2. Restriction digest
The isolated Pqrr4+B0034 plasmid was then cut with XbaI and PstI and ran on a 2% gel at 90V in order to verify the presence of the circuit. The following is an image of the gel:
The lower bands of the colonies 1, 2, 4, 6, 8, 9, and 10 seem to be above the positive control bands, which is what was expected. Thus it is now ready for sequencing.
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MANDY
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PATRICK
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PRIMA
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STEFAN
Meeting at Christian's lab
Today was full of wonderful manual labour as you can probably tell
from everyone's update. After that, I managed to read a Second Life
ethics paper. I didn't think it was very good because it was poorly
written and it seemed the person was not qualified to do it. However,
I enjoyed the paper with the molecular orbitals. I think it is the
same person that did the molecule rezzer as well. The paper showed how
useful Second Life can be tovisually present something, which is
excellent because synthetic biology is not very friendly to the naked
eye. I also started to write the ethics blog post and put some thought into
what to present on Friday.
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VICKI
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