From 2009.igem.org
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| - | Descriptive Title of What You're Doing
| + | Gel of the vector verification performed yesterday |
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| | <div class="desc"> | | <div class="desc"> |
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| - | WIKI CODING HERE
| + | Purpose: to visualise the results of yesterday’s vector verification |
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| | + | Expected restriction digest output: |
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| | + | Biobrick digest (2 fragments) |
| | + | * psB1AC3/psB1AK3: ~3.0 kb |
| | + | * ccdB (p1010): ~675 bp |
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| | + | TOPO digest (2 fragments) |
| | + | * TOPO (T/A and Blunt): ~3.5 kb |
| | + | * LuxOU: ~2.0 kb |
| | + | * LuxPQ: ~4.0 kb |
| | + | * LuxCDABE: ~6.0 kb |
| | + | * LuxOD47E: ~1.4 kb |
| | + | * LuxOD47A: ~1.4 kb |
| | + | |
| | + | Results: |
| | + | |
| | + | Gel and lane description is included below. LuxPQ did not work, so it will need to be sequenced. |
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| | + | Sequencing of LuxPQ in TOPO Blunt II |
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| | + | Purpose: As mentioned above, the results for LuxPQ are very questionable. Since they cannot be explained with restriction digests, hopefully sequencing will alleviate some of the confusion. |
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| | + | Materials and methods: |
| | + | We inserted 7.3 uL of LuxPQ in TOPO into a mini PCR tube so that around 730 ng of DNA were present for sequencing. We used the sp6 forward primer and the T7 reverse primer. |
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| | + | Results: |
| | + | Much like our previous sequencing attempts, these were questionable. The electronic copy will be inserted shortly. |
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Revision as of 22:41, 20 August 2009
University of Calgary
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CAROL
Descriptive Title of What You’re Doing
WIKI CODING HERE
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CHINMOYEE
More Simbiology
Exploration Results :
Simbiology assumes that all reactions are elementary reactions. For stochastic simulation only the Chemical kinetic and Hill kinetic equations work. Simbiology also produces a diagram of all the reactions input . The diagram view is helpfull in establishing the connections between species when they react or form.
The simbiology simulation graphs produced have a generic y-axis value of state which signifies concentration .
Started to look at other Toolboxes given with the Matlab package.
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EMILY
Descriptive Title of What You're Doing
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FAHD
Descriptive Title of What You're Doing
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IMAN
Descriptive Title of What You're Doing
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JAMIE
Descriptive Title of What You're Doing
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JEREMY
Descriptive Title of What You're Doing
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KATIE
Descriptive Title of What You're Doing
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KEVIN
Cloning Technique
Began looking into biobrick cloning techniques. Reviewed how the parts are put together. If A part is the recepient and B part is the donor, and when B part needs to be put behind of A, the recepient A is cut with SpeI and PstI sites and the donor is cut with XbaI and PstI. When B needs to be put in front of A, the recepient A is cut with EcoRI and Xba, and the donor B is cut with EcoRI and SpeI. When cut Xba and Spe sites meet, it creates a scar, making the construction permenant. (It won't cut apart with the enzymes we use)
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MANDY
Descriptive Title of What You're Doing
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PATRICK
Descriptive Title of What You're Doing
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PRIMA
Descriptive Title of What You're Doing
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STEFAN
Descriptive Title of What You're Doing
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VICKI
Gel of the vector verification performed yesterday
Purpose: to visualise the results of yesterday’s vector verification
Expected restriction digest output:
Biobrick digest (2 fragments)
- psB1AC3/psB1AK3: ~3.0 kb
- ccdB (p1010): ~675 bp
TOPO digest (2 fragments)
- TOPO (T/A and Blunt): ~3.5 kb
- LuxOU: ~2.0 kb
- LuxPQ: ~4.0 kb
- LuxCDABE: ~6.0 kb
- LuxOD47E: ~1.4 kb
- LuxOD47A: ~1.4 kb
Results:
Gel and lane description is included below. LuxPQ did not work, so it will need to be sequenced.
Sequencing of LuxPQ in TOPO Blunt II
Purpose: As mentioned above, the results for LuxPQ are very questionable. Since they cannot be explained with restriction digests, hopefully sequencing will alleviate some of the confusion.
Materials and methods:
We inserted 7.3 uL of LuxPQ in TOPO into a mini PCR tube so that around 730 ng of DNA were present for sequencing. We used the sp6 forward primer and the T7 reverse primer.
Results:
Much like our previous sequencing attempts, these were questionable. The electronic copy will be inserted shortly.
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