From 2009.igem.org
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- | Descriptive Title of What You're Doing
| + | Sequencing of what I hope will be LuxOD47A BBK |
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- | WIKI CODING HERE
| + | I prepared a sample of the recently-formed-and-colony-PCR'd-and-NotI-restriction-digested LuxOD47A BBk for sequencing. This was done with BBk sequencing primers, in accordance with the sequencing protocol. |
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Revision as of 23:07, 20 August 2009
University of Calgary
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CAROL
Descriptive Title of What You’re Doing
WIKI CODING HERE
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CHINMOYEE
CLASS
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EMILY
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FAHD
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IMAN
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JAMIE
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JEREMY
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KATIE
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KEVIN
1. Glycerol Stock
Glycerol stocks of R0040, B0015 J13002, and Pqrr4 were made in order to preserve them long term. The addition of glycerl is important because otherwise the cellular membrane would shear.
2. Plasmid Isolation
To obtain more isolated R0040, B0015, J1302, and Pqrr4, miniprep (plasmid isolation) was done on them.
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MANDY
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PATRICK
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PRIMA
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STEFAN
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VICKI
Sequencing of what I hope will be LuxOD47A BBK
I prepared a sample of the recently-formed-and-colony-PCR'd-and-NotI-restriction-digested LuxOD47A BBk for sequencing. This was done with BBk sequencing primers, in accordance with the sequencing protocol.
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