From 2009.igem.org
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| - | WIKI CODING HERE
| + | Isolated plasmid from LuxOD47E overnight cultures using Sigma miniprep kit, lot # 035K6068, eluting in ddH2O. Learned how to use Nanodrop Spectrophotometer to determine the concentration of the DNA. Prepared DNA for DNA sequecning. The optimal concentration of DNA for DNA sequencing is 100 ng/ 1 kb. Our gene is 1362 base pairs and the concentration is 292.3 ng/uL, so we sent down 1.8 uL of DNA with 7.2 uL ddH2O. |
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Revision as of 03:19, 21 August 2009
University of Calgary
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CAROL
Descriptive Title of What You’re Doing
WIKI CODING HERE
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CHINMOYEE
CLASS
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EMILY
Isolating Plamid, Nanodrop and Preparations for DNA Sequencing
Isolated plasmid from LuxOD47E overnight cultures using Sigma miniprep kit, lot # 035K6068, eluting in ddH2O. Learned how to use Nanodrop Spectrophotometer to determine the concentration of the DNA. Prepared DNA for DNA sequecning. The optimal concentration of DNA for DNA sequencing is 100 ng/ 1 kb. Our gene is 1362 base pairs and the concentration is 292.3 ng/uL, so we sent down 1.8 uL of DNA with 7.2 uL ddH2O.
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FAHD
Descriptive Title of What You're Doing
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IMAN
Descriptive Title of What You're Doing
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JAMIE
Descriptive Title of What You're Doing
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JEREMY
Descriptive Title of What You're Doing
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KATIE
Do Not Group Deed Objects
Lost my entire PCR machine script included today and will have to start over since no one is able to access anything within the object. I have old versions of the scripts I was using so hopefully I will be able to update them quickly and integrate all scripts together since it is really not practical and I will also need to add more types of DNA. This will most likely involve:
- Adding a variable to keep track of what type of DNA has been added so that the correct product may be given to the avatar later
- Making the different products and putting them into the machine’s inventory
- Adding the names of the different types of DNA to a list that will be searched through to see what exists and what doesn’t
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KEVIN
Plasmid Isolation (miniprep)
The pBluescript were isolated from TOP10 cells by using Sigma's GenElute plasmid Miniprep kit (sigma). The pBluescript is needed to verify more competent cells in the future.
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MANDY
Descriptive Title of What You're Doing
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PATRICK
Descriptive Title of What You're Doing
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PRIMA
Descriptive Title of What You're Doing
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STEFAN
Descriptive Title of What You're Doing
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VICKI
LuxOD47A plasmid isolation and preparation for sequencing
Plasmid isolation of competent LuxOD47A BBk cells
Purpose: to isolate plasmids from LuxOD47A cells that were cultured over the weekend
Protocol: refer to protocol page.
Concentration measurement of isolated plasmids
Purpose: to measure the concentration of the isolated plasmids with the Nanodrop spectrophotometer
Protocol: refer to protocol page.
Preparation of LuxOD47A in TOPO for sequencing
Protocol: refer to protocol page. 500 ng of DNA were inserted into the tube sent down for sequencing.
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