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Team:Calgary/24 July 2009
From 2009.igem.org
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| - | + | Marketing & RD of PQ-B-R-OU-B | |
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| - | + | Today: | |
| + | In Marketing: | ||
| + | I called Courtyard Marriot Hotel in Boston. They offered 279/night with 2 queen beds per room. I wanted to know if they offer any quotes for students but apparently Marriot hotels, in general, don’t offer any sort of quotes/discounts. | ||
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| + | I mailed the sponsorship pkg to GSK (GlaxoSmithKline) just as the Charitable Donations lady asked me to. Although she didn’t think iGEM’s goal this year matches the company’s objectives but she’s still willing to have a look at it. | ||
| + | I didn’t call any companies today because I’ve been continuously calling them for the past few weeks. I’ll do it Monday though. | ||
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| + | Company 1’s donation committee reviewed our pkg and said that since that company does not have a presence in Calgary and they only support communities where they have operations so they declined our proposal. I sent her a thank you letter and asked for contacts. | ||
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| + | In lab: | ||
| + | (shadowing Jeremy) I made a 0.7% agarose gel for restriction digest of Colony 6 and colony 7. (we took C6 +7 from a plasmid PCR that was done earlier by Jeremy to verify the presence of LuxPQ-B-R-OU-B in AC plasmid. C6 +7 looked the most accurate after Jeremy ran it on a gel so we used it for the restriction digest today. ) | ||
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| + | The purpose of this restriction digest was to verify the size of the PQ-B-R-OU-B. He let me do almost everything on my own while he was there. After we ran it on a gel, wells 2 and 4 (cut) was blank but the wells 3 and 5 (uncut) was clearly visible. We assumed there must’ve been a problem with the RD perhaps so we redid everything again. I made the gel again, etc. Yesterday, Jeremy did a RD of luxPQ and ccdb and left it overnight. Today we did a plasmid switch to transfer PQ from AK to AC. We anarctic phosphatsed it and ligated it, then we did a transformation to transfer PQ plasmid into top 10 cells. Finally we plated it on Chlroramphenical plates and left it overnight. | ||
| + | The rest of the time was spent in the ethics meeting this morning and major lab clean up in the afternoon. | ||
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Revision as of 06:39, 21 August 2009
UNIVERSITY OF CALGARY
