|
|
| Line 453: |
Line 453: |
| | <br> | | <br> |
| | <div class="heading"> | | <div class="heading"> |
| - | Second Fundraising Event
| + | Understanding Lab and Continuation with marketing |
| | </div> | | </div> |
| | <br> | | <br> |
| | <div class="desc"> | | <div class="desc"> |
| | </html> | | </html> |
| - | This was iGEM Calgary's Second bake sale. The team divided up the task of baking goodies. We met up at Emily's house yesterday to bake cupcakes, muffins, cakes and much more. We also had cinnamon buns, cheesecakes, samosas, cookies and the list goes on. I booked the Hippoccrates site the week before. I had to be at the sale all day, heating up food, handing out the goods and keeping track of the float. I also helped to clean up at the end of the event. we successfully raised $286.21.
| + | |
| | + | I learned the purpose and procedures for restriction digest from shadowing Jeremy. After hanging out with in the lab for some time, I began understanding what PCR was used for, when PCR needed to be done, what's the water bath for and much more. |
| | + | |
| | + | Carol also taught me what phosphatase is, when it should be done and even showed me how she was doing it. |
| | + | I kept following-up with the list of companies that Fahd and I had made in the beginning of the year. Many companies have turned down our proposals due to financial constraints. On the bright side, we decided to target mostly pharmaceutical and biotech companies, many of which are considering our sponsorship proposal. |
| | | | |
| | <html> | | <html> |
|
|
CAROL
Descriptive Title of What You’re Doing
WIKI CODING HERE
|
|
|
CHINMOYEE
Research more on Stochasticity
Looked into Papers :
Imapct of Stochasticity on gene regulatory networks
Analysis of Noise in AI-2 Circuits
Stochasticity in Transcriptional Regulation: Origins, Consequences,and Mathematical Representations
|
|
|
EMILY
Descriptive Title of What You're Doing
- Purpose: To verify that BBK LuxOD47E has been successfully cloned into the psB1AC3 vector.
- Performed a Colony PCR on LuxOD47E in psB1AC3 vector colonies from last week. Only digests done with XbaI / PstI enzymes produced colonies on plates, so these were the only colonies used.
- Used p Taq and LuxO forward and reverse primers. Cycling conditions were as follows: 94 C for 6 minutes, 36x (94 C for 30 sec, 55 C for 45 sec, 72 C for 90 sec), 72 C for 10 minutes and hold at 4 C.
- See gel photo below. Lanes 1-6 are LuxOD47E cut with XbaI / PstI, colonies 1-6, Lane 7 is a ngeative control with ddH2O.
- Results: Nothing was amplified. This was because I used the wrong buffer. Will proceed by re-running the Colony PCR with the CORRECT buffer (10x PCR Buffer- Cl2).
- Performed colony PCR again with the proper PCR Buffer this time. PCR products were visualized on a 1% agarose gel run at 120 V with Generuler 1.0 KB+ DNA Ladder. Lanes 1-7 are LuxOD47E in psB1AC3 cut with XbaI / PstI. Lane 8 is a negative control with only Mastermix + ddH2O.
- See gel photo below.
- From this gel, it looks like LuxOD47E is in the vector as we see the approproate band size of approximately 1.4 KB.
- Prepared overnight cultures of the colonies, will isolate plasmid tomorrow.
|
|
|
FAHD
Descriptive Title of What You're Doing
|
|
|
IMAN
Descriptive Title of What You're Doing
|
|
|
JAMIE
Descriptive Title of What You're Doing
|
|
|
JEREMY
Descriptive Title of What You're Doing
|
|
|
KATIE
Descriptive Title of What You're Doing
|
|
|
KEVIN
Restreak
Restreak of R0040, B0015, and J13002 were done to grow more of verified single colonies.
|
|
|
MANDY
Descriptive Title of What You're Doing
|
|
|
PATRICK
Descriptive Title of What You're Doing
|
|
|
PRIMA
Understanding Lab and Continuation with marketing
I learned the purpose and procedures for restriction digest from shadowing Jeremy. After hanging out with in the lab for some time, I began understanding what PCR was used for, when PCR needed to be done, what's the water bath for and much more.
Carol also taught me what phosphatase is, when it should be done and even showed me how she was doing it.
I kept following-up with the list of companies that Fahd and I had made in the beginning of the year. Many companies have turned down our proposals due to financial constraints. On the bright side, we decided to target mostly pharmaceutical and biotech companies, many of which are considering our sponsorship proposal.
|
|
|
STEFAN
Descriptive Title of What You're Doing
|
|
|
VICKI
Descriptive Title of What You're Doing
|
"
