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CAROL
Descriptive Title of What You’re Doing
WIKI CODING HERE
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CHINMOYEE
Final Exam
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EMILY
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FAHD
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IMAN
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JAMIE
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JEREMY
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KATIE
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KEVIN
Researching for a possible candidate of our response circuit
Mandy and I were assigned as partners and we were able to find a possible application of our signalling circuit involving the antifreeze proteins. There are variety of organisms that express Antifreeze proteins (AFPs), including fish (Arctic cod), bacteria (Pseudomonas putida), insects, and plants. Since we only work with bacteria, we attempted to find the most suitable antifreeze protein, and the antifreeze protein in Marinomonas primoryensis seemed to be most ideal.
Unlike other plant and bacterial antifreeze proteins, it is found to be hyperactive, meaning it is stable in low temperatures. Also, this antifreeze protein, like the one found in animals, utilizes a freeze-avoidant strategy which depresses the freezing point of the cell, while antifreeze proteins from other plants and bacteria utilize freeze-tolerant method, which does let the cell freeze, but it inhibits the growth of ice crystals; thus preventing the cell from damage.
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MANDY
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PATRICK
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PRIMA
Second Bake sale
This was iGEM Calgary's Second bake sale. The team divided up the task of baking goodies. We met up at Emily's house yesterday to bake cupcakes, muffins, cakes and much more. We also had cinnamon buns, cheesecakes, samosas, cookies and the list goes on. I booked the Hippoccrates site the week before. I had to be at the sale all day, heating up food, handing out the goods and keeping track of the float. I also helped to clean up at the end of the event. we successfully raised $370.00.
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STEFAN
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VICKI
Plasmid isolation of overnight cultures and subsequent NotI restriction digest
WIKI CODING HERE
Purpose: to confirm if a construct of the appropriate size can be excised from the plasmids transformed into TOP10 competent cells
Materials and methods
The plasmid isolation and restriction digest were performed in accordance with the procedure outlined on the protocol page. We expect to see 2 pieces from the J13002 + LuxOD47A BBk, one at 1685 bp and one at 3055 bp (for the AC plasmid); 2 pieces from the LuxOD47A BBk + B0015, one at 1750 bp and one at 3189 bp (for the AK plasmid); and 2 pieces from the LuxOD47A BBk size control, one at 1610 bp and one at 3055 bp (for the AC plasmid).
Results
Attached below. Lane 1 is the DNA ladder; lanes 2 and 3 are J13002 + LuxOD47A BBk (colonies 2 and 5 respectively); lane 4 is LuxOD47A BBk size control; lanes 5 and 6 are LuxOD47A BBk + B0015 (colonies 1 and 4 respectively); and lane 7 is a DNA ladder. The DNA ladder used was the GeneElute 1kb+. The bands for the mutant and mutant + B0015 are in line with what we expected.
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