Template:Team:KULeuven/14 August 2009/VanillinReceptor

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(Difference between revisions)
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# TOPO from W and Z and again from A
# TOPO from W and Z and again from A
#* Note: if A does not succes again (with the iGEM protocol) then proceed with a new PCR purification.
#* Note: if A does not succes again (with the iGEM protocol) then proceed with a new PCR purification.
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# Mutagenisis 1 on position 127 of R was performed

Revision as of 13:05, 25 August 2009

  1. G 2,3,1' on gel electrophorese
    • 2 and 3 look oke, 1000bp
    • 1' looks oke
    • 800bp fragment + 200bp TOPO)
  2. Innoculate 2 and 3 and grow on....
  3. TOPO from W and Z and again from A
    • Note: if A does not succes again (with the iGEM protocol) then proceed with a new PCR purification.
  4. Mutagenisis 1 on position 127 of R was performed
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