"
Template:Team:KULeuven/25 August 2009/BlueLightReceptor
From 2009.igem.org
(Difference between revisions)
(New page: # miniprepping the liquid cultures with pSB3K3 {| border ="1" align="center" !| Part || concentration (ng/μl) || 260/280 λ || |- align="center" | pSB3K3 (1) || 27,6 || 1,91 || ...) |
|||
| Line 1: | Line 1: | ||
| - | # miniprepping the liquid cultures with pSB3K3 | + | #* miniprepping the liquid cultures with pSB3K3 |
{| border ="1" align="center" | {| border ="1" align="center" | ||
!| Part || concentration (ng/μl) || 260/280 λ || | !| Part || concentration (ng/μl) || 260/280 λ || | ||
| Line 7: | Line 7: | ||
| pSB3K3 (2) || 47,7 || 2,08 || | | pSB3K3 (2) || 47,7 || 2,08 || | ||
|} | |} | ||
| + | #*restriction digest of pSB3K3 with EcoRI and PstI | ||
| + | #*gel electroforesis and extraction of the RD of pSB3K3 | ||
| + | --> failed. there was no signal at all | ||
| + | #*new colonies were ented in liquid culture. | ||
| + | #*ligation C performed: J23101 (A) + E0240 | ||
Revision as of 13:47, 25 August 2009
- miniprepping the liquid cultures with pSB3K3
| Part | concentration (ng/μl) | 260/280 λ | |
|---|---|---|---|
| pSB3K3 (1) | 27,6 | 1,91 | |
| pSB3K3 (2) | 47,7 | 2,08 |
- restriction digest of pSB3K3 with EcoRI and PstI
- gel electroforesis and extraction of the RD of pSB3K3
--> failed. there was no signal at all
- new colonies were ented in liquid culture.
- ligation C performed: J23101 (A) + E0240
