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August/24 August 2009
From 2009.igem.org
(Difference between revisions)
(New page: 1. '''LB+Amp plate''' <br> <br> 2.'''Min prep'''<br> Result of Nanodrop concentration check<br> (sample No.) (concentration) 7-1 27.8 ng/uL 8-1 23.2 ...) |
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8-1 23.2 | 8-1 23.2 | ||
| - | 9 | + | 9 21.1 |
10-A 29.1 | 10-A 29.1 | ||
10-B 25.1 | 10-B 25.1 | ||
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11(p(tetR)+RBS+GFP+terminater) | 11(p(tetR)+RBS+GFP+terminater) | ||
11-B OK | 11-B OK | ||
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| + | No. 9 10 → ligation rapid check | ||
Revision as of 04:08, 26 August 2009
1. LB+Amp plate
2.Min prep
Result of Nanodrop concentration check
(sample No.) (concentration)
7-1 27.8 ng/uL
8-1 23.2
9 21.1
10-A 29.1
10-B 25.1
11-A 15.7
11-B 34.1
3.transformed check
Checked the cell cultures transformed and plated out yesterday (8/23) for colony formation and made an approximate count of the number of colonies.
(sample No.) (no. of colonies)
1-4E ~5
1-8E 0
2-16L 1
2-16N ~15
2-18H 2
ligation sample
12 0
13 0
14 0
15 0
5 0
to iquid medium(5mL + Amp 5uL)
4.ligation check
| sample | 5 |
| XbaI | 1 |
| PstI | 1 |
| No.2 | 2 |
| dH2O | 11 |
| total | 20uL |
↓
37 , 2hr
↓
gel electrophoresis
result
7 (RBS+cinR)
7-1 OK
8 (RBS+LuxI)
8-1 OK
9 (RBS+LasR+terminater)
9 fialure?
10 (RBS+EspE+terminater)
10 ???
11(p(tetR)+RBS+GFP+terminater)
11-B OK
No. 9 10 → ligation rapid check
