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Template:Team:KULeuven/18 August 2009/VanillinReceptor
From 2009.igem.org
(Difference between revisions)
(New page: * PCR purification miniprep and nanodrop * start ethanol precipitatie (overnight ethanol + sodium acetate) * PCR of the fragements of TOPO (W and Z) was done to check if TOPO worked. Gelel...) |
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| - | * PCR purification miniprep and nanodrop | + | * PCR purification miniprep and nanodrop of R on mutation 2 |
| - | * start ethanol precipitatie (overnight ethanol + sodium acetate) | + | * start ethanol precipitatie (overnight ethanol + sodium acetate)to create a better result(more purified product) of product R with mutation 202 |
* PCR of the fragements of TOPO (W and Z) was done to check if TOPO worked. Gelelectroforesis showed nothing so TOPO cloning failed again for virA. | * PCR of the fragements of TOPO (W and Z) was done to check if TOPO worked. Gelelectroforesis showed nothing so TOPO cloning failed again for virA. | ||
Revision as of 12:46, 26 August 2009
- PCR purification miniprep and nanodrop of R on mutation 2
- start ethanol precipitatie (overnight ethanol + sodium acetate)to create a better result(more purified product) of product R with mutation 202
- PCR of the fragements of TOPO (W and Z) was done to check if TOPO worked. Gelelectroforesis showed nothing so TOPO cloning failed again for virA.
