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Template:Team:KULeuven/26 August 2009/BlueLightReceptor
From 2009.igem.org
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#* they will not be exposed to the light as long anymore. we decided that 1h will be more then enough. | #* they will not be exposed to the light as long anymore. we decided that 1h will be more then enough. | ||
#* possible bleaching? | #* possible bleaching? | ||
| - | # a colony from all three plates with the ligA-construct were taken and ented in liquid culture. this was needed to check wether the colonies were still. | + | # a colony from all three plates with the ligA-construct were taken and ented in liquid culture. this was needed to check wether the colonies were still alive. |
| + | #* culture 13/08 | ||
| + | #* culture 14/08 (1) | ||
| + | #* culture 14/08 (2) | ||
# by 5pm, one of the liquid cultures had grown and was miniprepped. 20μl was sent for sequencing together with 5μM of primer 2260. | # by 5pm, one of the liquid cultures had grown and was miniprepped. 20μl was sent for sequencing together with 5μM of primer 2260. | ||
{| border ="1" align="center" | {| border ="1" align="center" | ||
Revision as of 08:18, 27 August 2009
- the plates with ligation A (blp + GFP) where fetched from the blue light installation. there was no GFP signal. the following actions will be taken:
- the plasmids will be purified from the colonies and will be sequenced using primer 2260.
- next time we will probably put them in liquid cultures under the LEDs while shaking gently. also, other parameters that need to be considered are being researched.
- they will not be exposed to the light as long anymore. we decided that 1h will be more then enough.
- possible bleaching?
- a colony from all three plates with the ligA-construct were taken and ented in liquid culture. this was needed to check wether the colonies were still alive.
- culture 13/08
- culture 14/08 (1)
- culture 14/08 (2)
- by 5pm, one of the liquid cultures had grown and was miniprepped. 20μl was sent for sequencing together with 5μM of primer 2260.
| Part | concentration (ng/μl) | 260/280 λ | |
|---|---|---|---|
| ligA (14/08 (1)) | 23,1 | 2,21 |
4. two electroporations were performed. one with ligC (J23101 + E0240) and one with pSB3K3 DNA.
