Team:KULeuven/25 August 2009

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Revision as of 11:05, 27 August 2009

Project progress

Progress of parts

[edit] Blue Light Receptor

  • Miniprepping the liquid cultures with
Part concentration (ng/μl) 260/280 λ
(1) 27,6 1,91
(2) 47,7 2,08
  • Restriction digest of with EcoRI and PstI
  • Gel electrophoresis and extraction of the RD of

--> failed. There was no signal at all

  • New colonies were ented in liquid culture.
  • Ligation C performed: (A) +
Vector insert
+
30ng -> 1,4μl 40,8ng -> 2μl

[edit] Vanillin Production

  • We have colonies!!
  • Lots of EF, enough of SAMS
  • We made fluid cultures and let them grow overnight

[edit] Vanillin Receptor

  • PCR mutagenesis from R on 427, PCR purification and gelelectrophoresis: no signal on the picture probably because enting didn't show satisfying results
  • Ligation van X and Y in K14 at 16°C
  • New enting of R202
  • W has been ented on solid agar, so miniprep and nanodrop are scheduled for today
  • Mutagenesis of W + PCR purification

[edit] Key/Lock/Anti-Key

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