Template:Team:KULeuven/26 August 2009/BlueLightReceptor

(Difference between revisions)

Revision as of 20:47, 27 August 2009

the plates with ligation A (blp + GFP) where fetched from the blue light installation. there was no GFP signal. the following actions will be taken:
- the plasmids will be purified from the colonies and will be sequenced using primer 2260.
- next time we will probably put them in liquid cultures under the LEDs while shaking gently. also, other parameters that need to be considered are being researched.
- they will not be exposed to the light as long anymore. we decided that 1h will be more than enough.
- possible bleaching?
a colony from all three plates with the ligA-construct were taken and ented in liquid culture. this was needed to check wether the colonies were still alive.
- culture 13/08
- culture 14/08 (1)
- culture 14/08 (2)
by 5pm, one of the liquid cultures had grown and was miniprepped. 20μl was sent for sequencing together with 5μM of primer 2260.

Part	concentration (ng/μl)	260/280 λ
ligA (14/08 (1))	23,1	2,21

4. two electroporations were performed and plated on LB medium. one with ligC ( + ) and one with pSB3K3 DNA.

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 #* the plasmids will be purified from the colonies and will be sequenced using primer 2260.
 #* next time we will probably put them in liquid cultures under the LEDs while shaking gently. also, other parameters that need to be considered are being researched.
-#* they will not be exposed to the light as long anymore. we decided that 1h will be more then enough.
+#* they will not be exposed to the light as long anymore. we decided that 1h will be more than enough.
 #* possible bleaching?
 # a colony from all three plates with the ligA-construct were taken and ented in liquid culture. this was needed to check wether the colonies were still alive.