Team:TUDelft/27 August 2009

From 2009.igem.org

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The overnight colonies were used for miniprep.
The overnight colonies were used for miniprep.
!Za, !Zb, !Zg and !Zh had the highest DNA concentrations. These were downstream cut and checked on 1% agarose gel for fragment size. Fragment should be 1934 bp.  
!Za, !Zb, !Zg and !Zh had the highest DNA concentrations. These were downstream cut and checked on 1% agarose gel for fragment size. Fragment should be 1934 bp.  
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[[Image:Saeed19082009wiki.jpg|600px]]
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{| border="1" align="center"
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| lane || Part || Expected Plasmid Size || Status
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|- align="center"
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| 1 || [http://www.eurogentec.com/EGT/Images/RESALES/Electrophoresis/Regular%20DNA%20Ladder/7-SmartLadder.jpg DNA Ladder] ||  ||
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|- align="center"
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| 2 || !Fr1n upstream  || 1900 || ok, but not needed
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|- align="center"
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| 3 || !Fr1n downstream || 1900 || ok
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|- align="center"
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| 4 || destination plasmid || 3000 || ok
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|}
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{{Template:TUDelftiGEM2009_end}}
{{Template:TUDelftiGEM2009_end}}

Revision as of 14:11, 28 August 2009


27 August 2009

Saeed

The overnight colonies were used for miniprep. !Za, !Zb, !Zg and !Zh had the highest DNA concentrations. These were downstream cut and checked on 1% agarose gel for fragment size. Fragment should be 1934 bp.

Saeed19082009wiki.jpg

lane Part Expected Plasmid Size Status
1 [http://www.eurogentec.com/EGT/Images/RESALES/Electrophoresis/Regular%20DNA%20Ladder/7-SmartLadder.jpg DNA Ladder]
2  !Fr1n upstream 1900 ok, but not needed
3  !Fr1n downstream 1900 ok
4 destination plasmid 3000 ok




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