Uppsala-Sweden/6 September 2009

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==Moving ON to fride==
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{{Uppsala-Sweden Template}}
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ON cultures for the lux system were moved to the iGEM fridge for MiniPrep tomorrow
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==Checking Plates==
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All plates show colonoies.
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After picking colonies -> fridge.
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--[[User:Karl.brune|Karl.brune]] 23:08, 5 September 2009 (UTC)
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==Picking Colonies==
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I picked colonies from plates into 10µl selective media.
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--[[User:Karl.brune|Karl.brune]] 23:08, 5 September 2009 (UTC)
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5 Colonies of pirA (AC3) -> pirA-X
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5 Colonies of b0034-pirA (AC3) -> RA-X
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5 Colonies of B0034-Yadh2-B0015 (AC3) -> RYB-X
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5 Colonies of B0015 (AC3) - T-X
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5 Colonies of R0011-B0034 (AK3) -> RR-X
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3 Colonies of E1A1-B0015 (AK3) -> E1A1X
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3 Colonies of E1A2-B0015 (AK3) -> E1A2X
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3 Colonies of E1A3-B0015 (AK3) -> E1A3X
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3 Colonies of E1A4-B0015 (AK3) -> E1A4X
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3 Colonies of E1B1-B0015 (AK3) -> E1B1X
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3 Colonies of E1B2-B0015 (AK3) -> E1B2X
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3 Colonies of E1B3-B0015 (AK3) -> E1B3X
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3 Colonies of E1B4-B0015 (AK3) -> E1B4X
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3 Colonies of E21-B0015 (AK3) -> E21X
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3 Colonies of E22-B0015 (AK3) -> E22X
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3 Colonies of E23-B0015 (AK3) -> E23X
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3 Colonies of E24-B0015 (AK3) -> E24X
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X= # of colony
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--[[User:Karl.brune|Karl.brune]] 23:08, 5 September 2009 (UTC)
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==Colony PCR==
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TAQ colony PCR was performed.
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10µl reaction, standart mix with verfication primer VF2 and VR.
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Initial Denat: 1min 30sec @94°C
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40Cylces
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Denaturation: 45 sec @94°C
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Annealing: 45 sec @50°C
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Elongation: 1min 45sec @ 72°C
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Return to Denat.
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Final Elongation: 10min @72°C
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Infinite storage at 4°C
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Colony tubes were moved to fridge after taking template for the PCR
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--[[User:Karl.brune|Karl.brune]] 23:08, 5 September 2009 (UTC)
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Revision as of 23:10, 5 September 2009




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