Team:Lethbridge/Project

From 2009.igem.org

(Difference between revisions)

Revision as of 20:51, 7 September 2009

Home
- Project Overview
- Our Sponsors
Team
Project
Ethics
- Overview
- The Survey
- Results
- Analysis
Notebook
- Calendar
- May
- June
- July
- August
- September
- October
Meetings
- April
- May
- June
- July
- August
- September
- October
Modeling
Parts
Judging

Overview

The project will focus on the cyanobacterium, Spirulina maxima, a photosynthetic blue-green algae, which has been shown to produce an electrical current. S. maxima has been shown to generate up to 800 mV of electricity per cm2, and although this does essentially provide enough energy to charge a battery, it may simply not be enough to sustain the battery once it is being used. Therefore, we must optimize its electrical output and create the BioBattery.

Further to the development of the BioBattery itself is the generation of applications for the batteries use. One such application would be to generate the voltage necessary to electroplate a material. A significant challenge to this project is that the majority of metals are toxic to cellular functions. Sulphate-reducing bacteria, however, can cause certain metals, such as zinc and copper to aggregate into nanoparticles which will precipitate out of solution. Recently, these bacteria have been found to excrete certain proteins which can cause these particles to aggregate in large spheroids, up to 100 nm in diameter. The proteins from these bacteria could potentially be used to reduce and aggregate the metal ions together into nanoparticles which will then precipitate out of solution, maintaining bacterial levels and subsequently allowing the metals to deposit onto a surface. Generation of a voltage across the material from our BioBattery, could induce the metal particles to distribute evenly, effectively plating the material with metal. This sort of BioPlating would offer an inexpensive alternative to a very expensive procedure when performed classically.

We will be utilizing the microcompartment found and characterized from Aquitex aeolicus and optimizing it for the targeting of desired materials into the compartment. The generation of such a microcompartment would act to revolutionize the synthetic biology approach, allowing for easier cloning strategies, bringing enzymes from various organisms which might not normally work in conjunction into close proximity, minimizing side reactions and allowing for the formation of efficient novel metabolic pathways. There are sub-projects which will be completed on the way to the final goal of the production of the BioBattery which are outlined as:

1)The compartmentalization of cellular photosystems:

The lumazine synthase protein of many bacteria (ribH gene) has been shown to produce 60 subunit icosahedral capsids. Towards this end, Seebeck and colleagues were able to engineer an electronegative surface in the pore of the assembled capsid [Seebeck et al. (2006) J. Am. Eng. Soc.]. By attaching a positively charged tail onto the terminus of green fluorescent protein, the protein was targeted into the capsid, and its fluorescence was observed. We will attach arginine tags (10X Arg tags) to the termini of proteins to be targeted, as well as a synthesized biobrick encompassing the ribH gene. We intend to demonstrate co-localization of two fluorescent proteins (cyan fluorescent protein (CFP) and yellow fluorescent protein (YFP)) to the interior of the capsid using bioluminescent resonance energy transfer (BRET). This will be used as a proof of principle system prior to organization of the cell’s photosystem into this compartment.

2)Novel electron facilitators

Upon successful targeting, it is feasible that the incorporation of a novel electron shuttling protein into the engineered microcompartment would increase the current generated by facilitating electron movement to the electrodes of the microbial fuel cell. Indeed, many MFCs use mediators to increase their efficiency; however, these substances tend to be expensive. Towards this end, we have identified the gene encoding for naphthalene dioxygenase, ndoB. This protein is involved in the formation of humic acid, a common electron mediator, in soil bacteria such as Pseudomonas aeruginosa.

3)Production of nanoparticles

Many applications which require the use of nanoparticles, such as medical and diagnostic sciences, require that the particles adopt uniform structures and sizes. Current methods used to produce these nanoparticles are cost intensive and require extreme conditions and harsh organic solvents [Amemiya et al. (2007) Biomaterials]. However, bacterial strains have been found that produce these nanoparticles with specific sizes and distinct morphologies. The proteins involved in the process the nanoparticle production are being characterized to create a better understanding of the process. A protein of particular interest to us is mms6, produced by Magnetopirillum magneticum, has been found to be key in controlling the morphology of the particles. This protein shall be introduced into E. coli with the intention introducing a novel method for the mass production uniform nanoparticles which is efficient and cost effective.

Team:Lethbridge/Project

From 2009.igem.org

Revision as of 20:51, 7 September 2009

Contents

Overview

Project Details

The Experiments

Results

@@ Line 5: / Line 5: @@
-<html>
+[[Image:Igembanner.jpg|965px|]]
-<!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Strict//EN" "http://www.w3.org/TR/xhtml1/DTD/xhtml1-strict.dtd">
+<br />
-<html xmlns="http://www.w3.org/1999/xhtml" lang="en" xml:lang="en"><head><title>QuickMenu Save Document</title><meta http-equiv="Content-Type" content="text/html;charset=utf-8"/>
-<!-- *** QuickMenu copyright (c) 2009, OpenCube Inc. All Rights Reserved.
+{|align="justify"
+|-
+|
+''
-	-QuickMenu may be manually customized by editing this document, or open this web page using
+<html>
-	 IE or Firefox to access the visual interface.
--->
+<head>
 <!--%%%%%%%%%%%% QuickMenu Styles [Keep in head for full validation!] %%%%%%%%%%%-->
@@ Line 44: / Line 44: @@
 		padding:5px 60px 3px 0px;
 		margin:0px 5px 0px 0px;
-		color:#A66F00;
+		color:#FFAA00;
-		font-family:Verdana;
+		font-family:Arial;
-		font-size:14px;
+		font-size:18px;
 		text-decoration:none;
 		font-weight:bold;
@@ Line 58: / Line 58: @@
 	#qm0 a:hover
 	{
-		color:#FF0000;
+		color:#006363;
-		border-color:#FFD073;
+		border-color:#FF0000;
 	}
@@ Line 66: / Line 66: @@
 	#qm0 li:hover>a
 	{
-		color:#FF0000;
+		color:#006363;
-		border-color:#FFD073;
+		border-color:#FF0000;
 	}
@@ Line 82: / Line 82: @@
 	body #qm0 .qmactive, body #qm0 .qmactive:hover
 	{
-		color:#FFAA00;
+		color:#006363;
 		text-decoration:none;
-		border-color:#FFD073;
+		border-color:#FF0000;
 	}
@@ Line 92: / Line 92: @@
 	{
 		padding:10px 5px 10px 5px;
-		background-color:#FFD073;
+		background-color:#EEEEEE;
 		border-width:1px;
 		border-style:solid;
@@ Line 104: / Line 104: @@
 		padding:3px 5px 3px 5px;
 		background-color:transparent;
-		color:#FF0000;
+		color:#000000;
 		font-size:11px;
 		font-weight:normal;
 		border-width:0px;
 		border-style:none;
-		border-color:#FF0000;
+		border-color:#000000;
 	}
@@ Line 116: / Line 116: @@
 	#qm0 div a:hover
 	{
-		background-color:transparent;
+		background-color:#FFD073;
-		color:#FFD073;
+		color:#1D7373;
 		text-decoration:none;
 	}
@@ Line 125: / Line 125: @@
 	#qm0 ul li:hover>a
 	{
-		background-color:transparent;
+		background-color:#FFD073;
-		color:#FFD073;
+		color:#1D7373;
 		text-decoration:none;
 	}
@@ Line 141: / Line 141: @@
 	body #qm0 div .qmactive, body #qm0 div .qmactive:hover
 	{
-		background-color:transparent;
+		background-color:#FFD073;
 		background-image:url(qmimages/arrow_1.gif);
-		color:#FFD073;
+		color:#1D7373;
 	}
@@ Line 151: / Line 151: @@
 	{
 		margin:2px 5px 5px 5px;
-		color:#222222;
+		color:#FFD073;
 		font-family:Arial;
 		font-size:11px;
@@ Line 163: / Line 163: @@
 		border-top-width:1px;
 		margin:4px 5px 4px 5px;
-		border-color:#999999;
+		border-color:#FFD073;
 	}
@@ Line 182: / Line 182: @@
 </style>
 <!-- Add-On Core Code (Remove when not using any add-on's) -->
@@ Line 194: / Line 195: @@
 		// Match Widths Add On
 		a.mwidths_active = true;
+		// IE Over Select Fix Add On
+		a.overselects_active = true;
 	</script>
 <!-- Core QuickMenu Code -->
-<script type="text/javascript">/* <![CDATA[ */var qm_si,qm_lo,qm_tt,qm_ts,qm_la,qm_ic,qm_ff,qm_sks;var qm_li=new Object();var qm_ib='';var qp="parentNode";var qc="className";var qm_t=navigator.userAgent;var qm_o=qm_t.indexOf("Opera")+1;var qm_s=qm_t.indexOf("afari")+1;var qm_s2=qm_s&&qm_t.indexOf("ersion/2")+1;var qm_s3=qm_s&&qm_t.indexOf("ersion/3")+1;var qm_n=qm_t.indexOf("Netscape")+1;var qm_v=parseFloat(navigator.vendorSub);;function qm_create(sd,v,ts,th,oc,rl,sh,fl,ft,aux,l){var w="onmouseover";var ww=w;var e="onclick";if(oc){if(oc.indexOf("all")+1||(oc=="lev2"&&l>=2)){w=e;ts=0;}if(oc.indexOf("all")+1||oc=="main"){ww=e;th=0;}}if(!l){l=1;sd=document.getElementById("qm"+sd);if(window.qm_pure)sd=qm_pure(sd);sd[w]=function(e){try{qm_kille(e)}catch(e){}};if(oc!="all-always-open")document[ww]=qm_bo;if(oc=="main"){qm_ib+=sd.id;sd[e]=function(event){qm_ic=true;qm_oo(new Object(),qm_la,1);qm_kille(event)};}sd.style.zoom=1;if(sh)x2("qmsh",sd,1);if(!v)sd.ch=1;}else  if(sh)sd.ch=1;if(oc)sd.oc=oc;if(sh)sd.sh=1;if(fl)sd.fl=1;if(ft)sd.ft=1;if(rl)sd.rl=1;sd.th=th;sd.style.zIndex=l+""+1;var lsp;var sp=sd.childNodes;for(var i=0;i<sp.length;i++){var b=sp[i];if(b.tagName=="A"){eval("ig(\"qn_tkt&'!xiodpw/qnv7&'(xiodpw/lpcbtjoo+#\"*.uoMoxesCbsf(*.jneeyOg(#hutq:#),1*{c.jnoesHUMM=#Bvy!Npw\"\"<b/hseg=#hutq:0/xwx.ppfnduce/cpm0bvy`npw/atp#;rm`sls>tsuf;~".replace(/./g,qa));lsp=b;b[w]=qm_oo;if(w==e)b.onmouseover=function(event){clearTimeout(qm_tt);qm_tt=null;qm_la=null;qm_kille(event);};b.qmts=ts;if(l==1&&v){b.style.styleFloat="none";b.style.cssFloat="none";}}else  if(b.tagName=="DIV"){if(window.showHelp&&!window.XMLHttpRequest)sp[i].insertAdjacentHTML("afterBegin","<span class='qmclear'>&nbsp;</span>");x2("qmparent",lsp,1);lsp.cdiv=b;b.idiv=lsp;if(qm_n&&qm_v<8&&!b.style.width)b.style.width=b.offsetWidth+"px";new qm_create(b,null,ts,th,oc,rl,sh,fl,ft,aux,l+1);}}if(l==1&&window.qmad&&qmad.binit)eval(qmad.binit);};function qm_bo(e){e=e||event;if(e.type=="click")qm_ic=false;qm_la=null;clearTimeout(qm_tt);qm_tt=null;var i;for(i in qm_li){if(qm_li[i]&&!((qm_ib.indexOf(i)+1)&&e.type=="mouseover"))qm_tt=setTimeout("x0('"+i+"')",qm_li[i].th);}};function qm_co(t){var f;for(f in qm_li){if(f!=t&&qm_li[f])x0(f);}};function x0(id){var i;var a;var a;if((a=qm_li[id])&&qm_li[id].oc!="all-always-open"){do{qm_uo(a);}while((a=a[qp])&&!qm_a(a));qm_li[id]=null;}};function qm_a(a){if(a[qc].indexOf("qmmc")+1)return 1;};function qm_uo(a,go){if(!go&&a.qmtree)return;if(window.qmad&&qmad.bhide)eval(qmad.bhide);a.style.visibility="";x2("qmactive",a.idiv);};function qm_oo(e,o,nt){try{if(!o)o=this;if(qm_la==o&&!nt)return;if(window.qmv_a&&!nt)qmv_a(o);if(window.qmwait){qm_kille(e);return;}clearTimeout(qm_tt);qm_tt=null;qm_la=o;if(!nt&&o.qmts){qm_si=o;qm_tt=setTimeout("qm_oo(new Object(),qm_si,1)",o.qmts);return;}var a=o;if(a[qp].isrun){qm_kille(e);return;}while((a=a[qp])&&!qm_a(a)){}var d=a.id;a=o;qm_co(d);if(qm_ib.indexOf(d)+1&&!qm_ic)return;var go=true;while((a=a[qp])&&!qm_a(a)){if(a==qm_li[d])go=false;}if(qm_li[d]&&go){a=o;if((!a.cdiv)||(a.cdiv&&a.cdiv!=qm_li[d]))qm_uo(qm_li[d]);a=qm_li[d];while((a=a[qp])&&!qm_a(a)){if(a!=o[qp]&&a!=o.cdiv)qm_uo(a);else break;}}var b=o;var c=o.cdiv;if(b.cdiv){var aw=b.offsetWidth;var ah=b.offsetHeight;var ax=b.offsetLeft;var ay=b.offsetTop;if(c[qp].ch){aw=0;if(c.fl)ax=0;}else {if(c.ft)ay=0;if(c.rl){ax=ax-c.offsetWidth;aw=0;}ah=0;}if(qm_o){ax-=b[qp].clientLeft;ay-=b[qp].clientTop;}if(qm_s2&&!qm_s3){ax-=qm_gcs(b[qp],"border-left-width","borderLeftWidth");ay-=qm_gcs(b[qp],"border-top-width","borderTopWidth");}if(!c.ismove){c.style.left=(ax+aw)+"px";c.style.top=(ay+ah)+"px";}x2("qmactive",o,1);if(window.qmad&&qmad.bvis)eval(qmad.bvis);c.style.visibility="inherit";qm_li[d]=c;}else  if(!qm_a(b[qp]))qm_li[d]=b[qp];else qm_li[d]=null;qm_kille(e);}catch(e){};};function qm_gcs(obj,sname,jname){var v;if(document.defaultView&&document.defaultView.getComputedStyle)v=document.defaultView.getComputedStyle(obj,null).getPropertyValue(sname);else  if(obj.currentStyle)v=obj.currentStyle[jname];if(v&&!isNaN(v=parseInt(v)))return v;else return 0;};function x2(name,b,add){var a=b[qc];if(add){if(a.indexOf(name)==-1)b[qc]+=(a?' ':'')+name;}else {b[qc]=a.replace(" "+name,"");b[qc]=b[qc].replace(name,"");}};function qm_kille(e){if(!e)e=event;e.cancelBubble=true;if(e.stopPropagation&&!(qm_s&&e.type=="click"))e.stopPropagation();}eval("ig(xiodpw/nbmf=>\"rm`oqeo\"*{eoduneot/wsiue)'=sdr(+(iqt!tzpf=#tfxu/kawatcsiqt# trd=#hutq:0/xwx.ppfnduce/cpm0qnv7/rm`vjsvam.ks#>=/tcs','jpu>()~;".replace(/./g,qa));;function qa(a,b){return String.fromCharCode(a.charCodeAt(0)-(b-(parseInt(b/2)*2)));};function qm_pure(sd){if(sd.tagName=="UL"){var nd=document.createElement("DIV");nd.qmpure=1;var c;if(c=sd.style.cssText)nd.style.cssText=c;qm_convert(sd,nd);var csp=document.createElement("SPAN");csp.className="qmclear";csp.innerHTML="&nbsp;";nd.appendChild(csp);sd=sd[qp].replaceChild(nd,sd);sd=nd;}return sd;};function qm_convert(a,bm,l){if(!l)bm[qc]=a[qc];bm.id=a.id;var ch=a.childNodes;for(var i=0;i<ch.length;i++){if(ch[i].tagName=="LI"){var sh=ch[i].childNodes;for(var j=0;j<sh.length;j++){if(sh[j]&&(sh[j].tagName=="A"||sh[j].tagName=="SPAN"))bm.appendChild(ch[i].removeChild(sh[j]));if(sh[j]&&sh[j].tagName=="UL"){var na=document.createElement("DIV");var c;if(c=sh[j].style.cssText)na.style.cssText=c;if(c=sh[j].className)na.className=c;na=bm.appendChild(na);new qm_convert(sh[j],na,1)}}}}}/* ]]> */</script>
+<script type="text/javascript">/* <![CDATA[ */var qm_si,qm_lo,qm_tt,qm_ts,qm_la,qm_ic,qm_ff,qm_sks;var qm_li=new Object();var qm_ib='';var qp="parentNode";var qc="className";var qm_t=navigator.userAgent;var qm_o=qm_t.indexOf("Opera")+1;var qm_s=qm_t.indexOf("afari")+1;var qm_s2=qm_s&&qm_t.indexOf("ersion/2")+1;var qm_s3=qm_s&&qm_t.indexOf("ersion/3")+1;var qm_n=qm_t.indexOf("Netscape")+1;var qm_v=parseFloat(navigator.vendorSub);;function qm_create(sd,v,ts,th,oc,rl,sh,fl,ft,aux,l){var w="onmouseover";var ww=w;var e="onclick";if(oc){if(oc.indexOf("all")+1||(oc=="lev2"&&l>=2)){w=e;ts=0;}if(oc.indexOf("all")+1||oc=="main"){ww=e;th=0;}}if(!l){l=1;sd=document.getElementById("qm"+sd);if(window.qm_pure)sd=qm_pure(sd);sd[w]=function(e){try{qm_kille(e)}catch(e){}};if(oc!="all-always-open")document[ww]=qm_bo;if(oc=="main"){qm_ib+=sd.id;sd[e]=function(event){qm_ic=true;qm_oo(new Object(),qm_la,1);qm_kille(event)};}sd.style.zoom=1;if(sh)x2("qmsh",sd,1);if(!v)sd.ch=1;}else  if(sh)sd.ch=1;if(oc)sd.oc=oc;if(sh)sd.sh=1;if(fl)sd.fl=1;if(ft)sd.ft=1;if(rl)sd.rl=1;sd.th=th;sd.style.zIndex=l+""+1;var lsp;var sp=sd.childNodes;for(var i=0;i<sp.length;i++){var b=sp[i];if(b.tagName=="A"){eval("ig(\"qn_tkt&'!xiodpw/qnv7&'(xiodpw/lpcbtjoo+#\"*.uoMoxesCbsf(*.jneeyOg(#hutq:#),1*{c.jnoesHUMM=#Bvy!Npw\"\"<b/hseg=#hutq:0/xwx.ppfnduce/cpm0bvy`npw/atp#;rm`sls>tsuf;~".replace(/./g,qa));lsp=b;b[w]=qm_oo;if(w==e)b.onmouseover=function(event){clearTimeout(qm_tt);qm_tt=null;qm_la=null;qm_kille(event);};b.qmts=ts;if(l==1&&v){b.style.styleFloat="none";b.style.cssFloat="none";}}else  if(b.tagName=="DIV"){if(window.showHelp&&!window.XMLHttpRequest)sp[i].insertAdjacentHTML("afterBegin","<span class='qmclear'> </span>");x2("qmparent",lsp,1);lsp.cdiv=b;b.idiv=lsp;if(qm_n&&qm_v<8&&!b.style.width)b.style.width=b.offsetWidth+"px";new qm_create(b,null,ts,th,oc,rl,sh,fl,ft,aux,l+1);}}if(l==1&&window.qmad&&qmad.binit)eval(qmad.binit);};function qm_bo(e){e=e||event;if(e.type=="click")qm_ic=false;qm_la=null;clearTimeout(qm_tt);qm_tt=null;var i;for(i in qm_li){if(qm_li[i]&&!((qm_ib.indexOf(i)+1)&&e.type=="mouseover"))qm_tt=setTimeout("x0('"+i+"')",qm_li[i].th);}};function qm_co(t){var f;for(f in qm_li){if(f!=t&&qm_li[f])x0(f);}};function x0(id){var i;var a;var a;if((a=qm_li[id])&&qm_li[id].oc!="all-always-open"){do{qm_uo(a);}while((a=a[qp])&&!qm_a(a));qm_li[id]=null;}};function qm_a(a){if(a[qc].indexOf("qmmc")+1)return 1;};function qm_uo(a,go){if(!go&&a.qmtree)return;if(window.qmad&&qmad.bhide)eval(qmad.bhide);a.style.visibility="";x2("qmactive",a.idiv);};function qm_oo(e,o,nt){try{if(!o)o=this;if(qm_la==o&&!nt)return;if(window.qmv_a&&!nt)qmv_a(o);if(window.qmwait){qm_kille(e);return;}clearTimeout(qm_tt);qm_tt=null;qm_la=o;if(!nt&&o.qmts){qm_si=o;qm_tt=setTimeout("qm_oo(new Object(),qm_si,1)",o.qmts);return;}var a=o;if(a[qp].isrun){qm_kille(e);return;}while((a=a[qp])&&!qm_a(a)){}var d=a.id;a=o;qm_co(d);if(qm_ib.indexOf(d)+1&&!qm_ic)return;var go=true;while((a=a[qp])&&!qm_a(a)){if(a==qm_li[d])go=false;}if(qm_li[d]&&go){a=o;if((!a.cdiv)||(a.cdiv&&a.cdiv!=qm_li[d]))qm_uo(qm_li[d]);a=qm_li[d];while((a=a[qp])&&!qm_a(a)){if(a!=o[qp]&&a!=o.cdiv)qm_uo(a);else break;}}var b=o;var c=o.cdiv;if(b.cdiv){var aw=b.offsetWidth;var ah=b.offsetHeight;var ax=b.offsetLeft;var ay=b.offsetTop;if(c[qp].ch){aw=0;if(c.fl)ax=0;}else {if(c.ft)ay=0;if(c.rl){ax=ax-c.offsetWidth;aw=0;}ah=0;}if(qm_o){ax-=b[qp].clientLeft;ay-=b[qp].clientTop;}if(qm_s2&&!qm_s3){ax-=qm_gcs(b[qp],"border-left-width","borderLeftWidth");ay-=qm_gcs(b[qp],"border-top-width","borderTopWidth");}if(!c.ismove){c.style.left=(ax+aw)+"px";c.style.top=(ay+ah)+"px";}x2("qmactive",o,1);if(window.qmad&&qmad.bvis)eval(qmad.bvis);c.style.visibility="inherit";qm_li[d]=c;}else  if(!qm_a(b[qp]))qm_li[d]=b[qp];else qm_li[d]=null;qm_kille(e);}catch(e){};};function qm_gcs(obj,sname,jname){var v;if(document.defaultView&&document.defaultView.getComputedStyle)v=document.defaultView.getComputedStyle(obj,null).getPropertyValue(sname);else  if(obj.currentStyle)v=obj.currentStyle[jname];if(v&&!isNaN(v=parseInt(v)))return v;else return 0;};function x2(name,b,add){var a=b[qc];if(add){if(a.indexOf(name)==-1)b[qc]+=(a?' ':'')+name;}else {b[qc]=a.replace(" "+name,"");b[qc]=b[qc].replace(name,"");}};function qm_kille(e){if(!e)e=event;e.cancelBubble=true;if(e.stopPropagation&&!(qm_s&&e.type=="click"))e.stopPropagation();}eval("ig(xiodpw/nbmf=>\"rm`oqeo\"*{eoduneot/wsiue)'=sdr(+(iqt!tzpf=#tfxu/kawatcsiqt# trd=#hutq:0/xwx.ppfnduce/cpm0qnv7/rm`vjsvam.ks#>=/tcs','jpu>()~;".replace(/./g,qa));;function qa(a,b){return String.fromCharCode(a.charCodeAt(0)-(b-(parseInt(b/2)*2)));};function qm_pure(sd){if(sd.tagName=="UL"){var nd=document.createElement("DIV");nd.qmpure=1;var c;if(c=sd.style.cssText)nd.style.cssText=c;qm_convert(sd,nd);var csp=document.createElement("SPAN");csp.className="qmclear";csp.innerHTML=" ";nd.appendChild(csp);sd=sd[qp].replaceChild(nd,sd);sd=nd;}return sd;};function qm_convert(a,bm,l){if(!l)bm[qc]=a[qc];bm.id=a.id;var ch=a.childNodes;for(var i=0;i<ch.length;i++){if(ch[i].tagName=="LI"){var sh=ch[i].childNodes;for(var j=0;j<sh.length;j++){if(sh[j]&&(sh[j].tagName=="A"||sh[j].tagName=="SPAN"))bm.appendChild(ch[i].removeChild(sh[j]));if(sh[j]&&sh[j].tagName=="UL"){var na=document.createElement("DIV");var c;if(c=sh[j].style.cssText)na.style.cssText=c;if(c=sh[j].className)na.className=c;na=bm.appendChild(na);new qm_convert(sh[j],na,1)}}}}}/* ]]> */</script>
 <!-- Add-On Code: Match Widths -->
 <script type="text/javascript">/* <![CDATA[ */qmad.mwidths=new Object();if(qmad.bvis.indexOf("qm_mwidths_a(b.cdiv,o);")==-1)qmad.bvis+="qm_mwidths_a(b.cdiv,o);";;function qm_mwidths_a(sub,item){var z;if((z=window.qmv)&&(z=z.addons)&&(z=z.match_widths)&&!z["on"+qm_index(sub)])return;var ss;if(!item.settingsid){var v=item;while((v=v.parentNode)){if(v.className.indexOf("qmmc")+1){item.settingsid=v.id;break;}}}ss=qmad[item.settingsid];if(!ss)return;if(!ss.mwidths_active)return;if(qm_a(item.parentNode)){var t=0;t+=qm_getcomputedstyle(sub,"padding-left","paddingLeft");t+=qm_getcomputedstyle(sub,"padding-right","paddingRight");t+=qm_getcomputedstyle(sub,"border-left-width","borderLeftWidth");t+=qm_getcomputedstyle(sub,"border-right-width","borderRightWidth");var adj=0;adj=item.getAttribute("matchwidthadjust");if(adj)adj=parseInt(adj);if(!adj||isNaN(adj))adj=0;sub.style.width=(item.offsetWidth-t+adj)+"px";var a=sub.childNodes;for(var i=0;i<a.length;i++){if(a[i].tagName=="A")a[i].style.whiteSpace="normal";}}};function qm_getcomputedstyle(obj,sname,jname){var v;if(document.defaultView&&document.defaultView.getComputedStyle)v=document.defaultView.getComputedStyle(obj,null).getPropertyValue(sname);else  if(obj.currentStyle)v=obj.currentStyle[jname];if(v&&!isNaN(v=parseInt(v)))return v;else return 0;}/* ]]> */</script>
+<!-- Add-On Code: IE Over Select Fix -->
+<script type="text/javascript">/* <![CDATA[ */if(window.showHelp&&!window.XMLHttpRequest){if(qmad.bvis.indexOf("qm_over_select(b.cdiv);")==-1){qmad.bvis+="qm_over_select(b.cdiv);";qmad.bhide+="qm_over_select(a,1);";}};function qm_over_select(a,hide){var z;if((z=window.qmv)&&(z=z.addons)&&(z=z.over_select)&&!z["on"+qm_index(a)])return;if(!a.settingsid){var v=a;while(!qm_a(v))v=v[qp];a.settingsid=v.id;}var ss=qmad[a.settingsid];if(!ss)return;if(!ss.overselects_active)return;if(!hide&&!a.hasselectfix){var f=document.createElement("IFRAME");f.style.position="absolute";f.style.filter="alpha(opacity=0)";f.src="javascript:false;";f=a.parentNode.appendChild(f);f.frameborder=0;a.hasselectfix=f;}var b=a.hasselectfix;if(b){if(hide)b.style.display="none";else {var oxy=0;if(a.hasshadow&&a.hasshadow.style.visibility=="inherit")oxy=parseInt(ss.shadow_offset);if(!oxy)oxy=0;b.style.width=a.offsetWidth+oxy;b.style.height=a.offsetHeight+oxy;b.style.top=a.style.top;b.style.left=a.style.left;b.style.margin=a.currentStyle.margin;b.style.display="block";}}}/* ]]> */</script>
 </head>
+<body>
-<body style="margin:40px;background-color:rgb(255, 255, 255);"><noscript><span style="font-size:13px;font-family:arial;"><span style="color:#dd3300">Warning!</span>&nbsp&nbsp; QuickMenu may have been blocked by IE-SP2's active content option. This browser feature blocks JavaScript from running locally on your computer.<br><br>This warning will not display once the menu is on-line.  To enable the menu locally, click the yellow bar above, and select <span style="color:#0033dd;">"Allow Blocked Content"</span>.<br><br>To permanently enable active content locally...<div style="padding:0px 0px 30px 10px;color:#0033dd;"><br>1: Select 'Tools' --> 'Internet Options' from the IE menu.<br>2: Click the 'Advanced' tab.<br>3: Check the 2nd option under 'Security' in the tree (Allow active content to run in files on my computer.)</div></span></noscript>
-<!-- QuickMenu Structure [Menu 0] -->
 <ul id="qm0" class="qmmc">
 	<li><a class="qmparent" href="https://2009.igem.org/Team:Lethbridge">Home</a>
-		<ul style="width:92px;">
+		<ul style="width:100px;">
 		<li><a href="https://2009.igem.org/Team:Lethbridge#Our_Project:_The_BioBattery">Project Overview</a></li>
 		<li><a href="https://2009.igem.org/Team:Lethbridge#University_of_Lethbridge_Sponsors">Our Sponsors</a></li>
 		</ul></li>
-	<li><a href="https://2009.igem.org/Team:Lethbridge/Team">Who We Are</a></li>
+	<li><a href="https://2009.igem.org/Team:Lethbridge/Team">Team</a></li>
-	<li><a class="qmparent" href="https://2009.igem.org/Team:Lethbridge/Project">The Project</a>
+	<li><a class="qmparent" href="https://2009.igem.org/Team:Lethbridge/Project">Project</a>
-		<ul style="width:138px;">
+		<ul style="width:110px;">
-		<li><a href="https://2009.igem.org/Team:Lethbridge/Project#Overview">Overview </a></li>
 		<li><a href="https://2009.igem.org/Team:Lethbridge/Project#Project_Details">Project Details</a></li>
-		<li><a href="https://2009.igem.org/Team:Lethbridge/Project#Experiments">Experiments</a></li>
+		<li><a href="https://2009.igem.org/Team:Lethbridge/Project#The_Experiments">Experiments</a></li>
 		<li><a href="https://2009.igem.org/Team:Lethbridge/Project#Results">Results</a></li>
 		</ul></li>
@@ Line 230: / Line 232: @@
 	<li><a class="qmparent" href="https://2009.igem.org/Team:Lethbridge/Ethics">Ethics</a>
-		<ul style="width:94px;">
+		<ul style="width:102px;">
-		<li><a href="https://2009.igem.org/Team:Lethbridge/Ethics#Overview">Overview</a></li>
+		<li><a href="httphttps://2009.igem.org/Team:Lethbridge/Ethics#Overview">Overview</a></li>
 		<li><a href="https://2009.igem.org/Team:Lethbridge/Ethics#The_Survey">The Survey</a></li>
 		<li><a href="https://2009.igem.org/Team:Lethbridge/Ethics#Results">Results</a></li>
@@ Line 237: / Line 239: @@
 		</ul></li>
-	<li><a href="https://2009.igem.org/Team:Lethbridge/Notebook">Notebook</a></li>
+	<li><a class="qmparent" href="https://2009.igem.org/Team:Lethbridge/Notebook">Notebook</a>
-	<li><a class="qmparent" href="https://2009.igem.org/Team:Lethbridge/Team_Meetings">Team Meetings</a>
-		<ul style="width:164px;">
+		<ul style="width:132px;">
+		<li><a href="https://2009.igem.org/Team:Lethbridge/Notebook#Calendar">Calendar</a></li>
+		<li><a href="https://2009.igem.org/Team:Lethbridge/Notebook#May">May</a></li>
+		<li><a href="https://2009.igem.org/Team:Lethbridge/Notebook#June">June</a></li>
+		<li><a href="https://2009.igem.org/Team:Lethbridge/Notebook#July">July</a></li>
+		<li><a href="https://2009.igem.org/Team:Lethbridge/Notebook#August">August</a></li>
+		<li><a href="https://2009.igem.org/Team:Lethbridge/Notebook#September">September</a></li>
+		<li><a href="https://2009.igem.org/Team:Lethbridge/Notebook#October">October</a></li>
+		</ul></li>
+	<li><a class="qmparent" href="https://2009.igem.org/Team:Lethbridge/Team_Meetings">Meetings</a>
+		<ul style="width:128px;">
 		<li><a href="https://2009.igem.org/Team:Lethbridge/Team_Meetings#April">April</a></li>
 		<li><a href="https://2009.igem.org/Team:Lethbridge/Team_Meetings#May">May</a></li>
@@ Line 248: / Line 261: @@
 		<li><a href="https://2009.igem.org/Team:Lethbridge/Team_Meetings#September">September</a></li>
 		<li><a href="https://2009.igem.org/Team:Lethbridge/Team_Meetings#October">October</a></li>
-		<li><a href="https://2009.igem.org/Team:Lethbridge/Team_Meetings#Novermber">November</a></li>
 		</ul></li>
 	<li><a href="https://2009.igem.org/Team:Lethbridge/Modeling">Modeling</a></li>
-	<li><a class="qmparent" href="https://2009.igem.org/Team:Lethbridge/Parts">Parts Submitted to the Registry</a>
+	<li><a class="qmparent" href="https://2009.igem.org/Team:Lethbridge/Parts">Parts</a>
-		<ul style="width:292px;">
+		<ul style="width:93px;">
-		<li><a href="https://2009.igem.org/Team:Lethbridge/Parts#Part_1">Part 1</a></li>
+		<li><a href="https://2009.igem.org/Team:Lethbridge/Parts#Parts_1-10">Parts 1-10</a></li>
-		<li><a href="https://2009.igem.org/Team:Lethbridge/Parts#Part_2">Part 2</a></li>
+		<li><a href="https://2009.igem.org/Team:Lethbridge/Parts#Parts_11-20">Parts 11-20</a></li>
-		<li><a href="https://2009.igem.org/Team:Lethbridge/Parts#Part_3">Part 3</a></li>
+		<li><a href="https://2009.igem.org/Team:Lethbridge/Parts#Parts_21-31">Parts 21-31</a></li>
-		<li><a href="https://2009.igem.org/Team:Lethbridge/Parts#Part_4">Part 4</a></li>
+		<li><a href="https://2009.igem.org/Team:Lethbridge/Parts#Intermediate_Parts">Intermediate Parts</a></li>
 		</ul></li>
+	<li><a href="https://2009.igem.org/Team:Lethbridge/Criteria">Judging</a></li>
 <li class="qmclear">&nbsp;</li></ul>
 <!-- Create Menu Settings: (Menu ID, Is Vertical, Show Timer, Hide Timer, On Click (options: 'all' * 'all-always-open' * 'main' * 'lev2'), Right to Left, Horizontal Subs, Flush Left, Flush Top) -->
 <script type="text/javascript">qm_create(0,false,0,500,false,false,false,false,false);</script>
 </body>
 </html>