From 2009.igem.org
(Difference between revisions)
|
|
| Line 1: |
Line 1: |
| - | {{UNIPV-Pavia/StyleCss}}
| |
| | <html> | | <html> |
| - | <head>
| + | |
| - | <link rel="stylesheet" type="text/css" href="http://bioinfo.unipv.it/igem/templates/SpryAssets/SpryMenuBar.js" title="Stile"/>
| + | |
| - | </head>
| + | |
| - | <body>
| + | |
| | <table width="100%"> | | <table width="100%"> |
| | <tr> | | <tr> |
| Line 12: |
Line 8: |
| | </tr> | | </tr> |
| | </table> | | </table> |
| - | </body>
| + | |
| | </html> | | </html> |
| | | | |
Revision as of 06:54, 8 September 2009
|
|
|
Protocols
|
|
|
Electrophoresis
(estimated time: 2 hours)
- Prepare agarose gel in 1x TBE buffer
- Add ethidium bromide (using gloves and face mask for your safety):
- 1 µl in the small size agarose gel (70 ml)
- 2 µl in the middle size agarose gel (150 ml)
- 4 µl in the big size agarose gel (250 ml)
- Cast the gel, insert the well-forming comb and let it polymerize
- Add the loading buffer to each sample
- Load the samples and 8 µl of marker
- Set to 70-100 volts and electrophorese for the required amount of time
- Use UV-light to look at the bands
- Take a picture of the gel, if needed (not when bands have to be cut!!!)
|
- DNA samples
- Ethidium bromide
- Loading buffer 10x Blue Juice, Invitrogen
- TBE (Tris/Borate/EDTA buffer) 5x (final volume 1 liter)
- 54 gr Tris
- 27.5 gr Borate
- 20 ml EDTA 0.5 M (pH 8)
- Marker (1 kb DNA Ladder, Promega)
- Face mask and gloves
- Electrophoresis apparatus
- Transilluminator
|
|
- NOTE: when not specified, the marker has the following pattern:
"
