Template:Team:KULeuven/24 August 2009/BlueLightReceptor

(Difference between revisions)

Revision as of 09:13, 8 September 2009

Plates with ligA were put under blue light. the LEDs were put on their max capacity.
Restriction digest with
- tubes (1,3,5,7,9) of ligA (BLP + ) cut with EcoRI en PstI
- promotor cut with EcoRI en SpeI (4x)
Gel electroforesis with the RD of ligA and followed by a gel extraction:
- Note: tube 5 of ligA was loaded poorly on the gel and could not be used.
- Note: the samples of promotor were barely visible. Only 2 of the 4 samples were recovered by extraction.

4. Enting of liquid cultures with kanamycin and

@@ Line 1: / Line 1: @@
-# plates with ligA were put under blue light. the LEDs were put on their max capacity.
+# Plates with ligA were put under blue light. the LEDs were put on their max capacity.
-# restriction digest with
+# Restriction digest with
 #*tubes (1,3,5,7,9) of ligA (BLP + {{kulpart|BBa_E0240}}) cut with EcoRI en PstI
 #*promotor {{kulpart|BBa_J23101}} cut with EcoRI en SpeI (4x)
-#gel electroforese with the RD of ligA and {{kulpart|BBa_J23101}} followed by a gel extraction:
+#Gel electroforesis with the RD of ligA and {{kulpart|BBa_J23101}} followed by a gel extraction:
-#* note: tube 5 of ligA was loaded poorly on the gel and could not be used.
+#* Note: tube 5 of ligA was loaded poorly on the gel and could not be used.
-#* note: the samples of promotor {{kulpart|BBa_J23101}} were barly visible. only 2 of the 4 samples were recoverd by extraction.
+#* Note: the samples of promotor {{kulpart|BBa_J23101}} were barely visible. Only 2 of the 4 samples were recovered by extraction.
 {| border ="1" align="center"
 !| Part || concentration (ng/&mu;l) || 260/280 &lambda; ||
@@ Line 21: / Line 21: @@
 | {{kulpart|BBa_J23101}} (B) ||12,8|| 2,57 ||
 |}
-. enting of liquid cultures with kanamycin and {{kulpart|pSB3K3}}
+. Enting of liquid cultures with kanamycin and {{kulpart|pSB3K3}}