From 2009.igem.org
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| - | Descriptive Title of What You’re Doing
| + | Multiple Miniprep isolations |
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| - | WIKI CODING HERE
| + | * Attempted 15 miniprep reactions on both non-mutated and mutated <i>luxCDABE</i> plasmid. |
| | + | * The isolated plasmid was pooled together and attempted to concentrate the plasmid by spinning the tube in a vacifuge. After several hours (2.5 hours) the concentration was higher. |
| | + | * Tried ethanol precipitation earlier in the day but there was no precipitate found when done. |
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Revision as of 05:22, 10 September 2009
University of Calgary
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CAROL
Multiple Miniprep isolations
- Attempted 15 miniprep reactions on both non-mutated and mutated luxCDABE plasmid.
- The isolated plasmid was pooled together and attempted to concentrate the plasmid by spinning the tube in a vacifuge. After several hours (2.5 hours) the concentration was higher.
- Tried ethanol precipitation earlier in the day but there was no precipitate found when done.
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CHINMOYEE
CLASS
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EMILY
Verification NotI Digest
- Objective: To do one last verification that LuxOD47E is in the psB1AC3 vector before we send it down for DNA Sequecning. We will do a NotI digest with
- Started with Plasmid Isolatation this morning (Sigma).
- Used 1000 Nanodrop Spectrophotometer to determine concentrations.
- Set up a restriction digest with NotI enzyme and REact Buffer 3, left to digest in the 37 C waterbath overnight.
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FAHD
Marketing for 23rd June 2009
Today, I helped finish the July newsletter which will be ready be next week to sent out to our sponsors. Also I e-mailed some research and development companies and will do a follow-up soon.
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IMAN
Descriptive Title of What You're Doing
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JAMIE
Descriptive Title of What You're Doing
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JEREMY
Isolating LuxPQ in psB1AK3 from overnights
Purpose: isolate LuxPQ in psB1AK3 from overnights of Colony 4 to continue construction of signaling circuit. Plasmid was isolated from colony 4 of luxPQ. This was done using the SIGMA’s Genelute plasmid Miniprep Kit. Plasmid purity and concentration were measured using the NanoDrop Spectrophotometer.
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KATIE
Descriptive Title of What You're Doing
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KEVIN
1. Glycerol Stock
Glycerol stocks of R0040, B0015 J13002, and Pqrr4 were made in order to preserve them long term. The addition of glycerl is important because otherwise the cellular membrane would shear.
2. Plasmid Isolation
To obtain more isolated R0040, B0015, J1302, and Pqrr4, miniprep (plasmid isolation) was done on them.
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MANDY
Descriptive Title of What You're Doing
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PATRICK
Descriptive Title of What You're Doing
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PRIMA
Marketing & Sponsorship
After constantly following up with companies over the past few weeks, I successfully recruited Alberta Research Council to sponsor the University of Calgary's iGEM team. They have generously donated %250 dollars this year.
We also edited the June newsletter and discussed it with the rest of the marketing team prior to sending it to all the companies we had contacted (including those who have declined our proposal).
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STEFAN
Descriptive Title of What You're Doing
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VICKI
Sequencing of what I hope will be LuxOD47A BBK
I prepared a sample of the recently-formed-and-colony-PCR'd-and-NotI-restriction-digested LuxOD47A BBk for sequencing. This was done with BBk sequencing primers, in accordance with the sequencing protocol.
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