Team:Chiba/Notebook/Calendar/9 September 2009

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[[Image:Chiba_Labwork_09Sep09.jpg|200px]] [[Image:Chiba_Labwork_09Sep09-2.jpg|200px]]
[[Image:Chiba_Labwork_09Sep09.jpg|200px]] [[Image:Chiba_Labwork_09Sep09-2.jpg|200px]]
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0800 main culture
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    divided injection 12.5ml of the culture to 10 50ml centrifuging tubes
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    WASH 3 times
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    Add 12.5ml new culture
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    Centrifuge the 1st tube 3000rpm 5min 20℃ and pick the upernatant
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    Do same operation every 15min to 2nd~10th tubes
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    after all, mix the supernatant and solid media at 1:1
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    make 10 concentration varieties of solid media

Revision as of 05:42, 10 September 2009

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(8_September_2009 <|>10_September_2009)


Chiba Labwork 09Sep09.jpg Chiba Labwork 09Sep09-2.jpg


0800 main culture 

    divided injection 12.5ml of the culture to 10 50ml centrifuging tubes 
    WASH 3 times
    Add 12.5ml new culture
  
    Centrifuge the 1st tube 3000rpm 5min 20℃ and pick the upernatant

    Do same operation every 15min to 2nd~10th tubes

    after all, mix the supernatant and solid media at 1:1
    make 10 concentration varieties of solid media
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