Revision as of 12:19, 10 September 2009

Used Parts
[http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2009&group=KULeuven Submitted Parts]

Individual parts

Short description	Resistance	Status	Location	Additional notes
Ribokey	A/A	ordered at GeneArt
antikey	A/A	ordered at GeneArt
lock	Ap	ordered at GeneArt
Terminator	Ak	Available here	-80	Can be found in -20 freezer
RBS	Ap	Available here	-80
promotor	Ap	Available here	-80	Can be found in -20 freezer
RBS + Sam8	A	send from Edinburgh	-20
Sam5	A	send from Edinburgh	-20
comt	A/A	send from Edinburgh/available in iGEM plates	-20
fcs	Ap	received from Edinburgh	-20
ech	Ap	received from Edinburgh	-20
VirA receptor		primers ordered		This is purified from Agrobacterium tumefaciens
VirG activator		primers ordered		This is purified from Agrobacterium tumefaciens
RpoA		primers ordered		This is purified from Agrobacterium tumefaciens and is necessary for VirG to work in E.coli
VirB promoter region		primers ordered		This is purified from Agrobacterium tumefaciens and has virboxes that bind VirG
YcgF/E promotor		region purified from E.coli strain MC4100	-20	blue light promotor region from http://www.ncbi.nlm.nih.gov/pubmed/19240136 1
YcgF/E promotor		in progress of being made		exact blue light promotor from http://www.ncbi.nlm.nih.gov/pubmed/19240136 1
plasmid backbone		from distribution plates		kanamycine resistant plasmid
GFP reporter		from last years glycerol stock		GFP+LVA+terminator
TetR generator		from last years glycerol stock		promotor+RBS+tetR+terminator
GFP reporter		from last years glycerol stock		RBS+GFP+terminator

Short description	Status	Additional notes
VirB promotor + key	Biobrick in registry
Blr promotor + key	Biobrick in registry
Vanillin receptor	Biobrick in registry
Vanillin synthesis	Biobrick in registry	5 paths in pathway never tested together in E. coli
Short Vanillin synthesis	Biobrick in registry	Short version containing the last 2 genes of the pathway, these have been tested in E. coli
Short Vanillin synthesis II	Biobrick in registry	Short version containing the first 3 parts of the pathway, these have not been tested in E. coli

@@ Line 13: / Line 13: @@
 | {{kulpart|BBa_J23078|icon}}|| lock || Ap || ordered at GeneArt ||  ||
 |-align="center"
-| {{kulpart|BBa_B0015|icon}}  ||Terminator|| Ak || Available here|| -80  || Terug te vinden in de -20
+| {{kulpart|BBa_B0015|icon}}  ||Terminator|| Ak || Available here|| -80  || Can be found in -20 freezer
 |-align="center"
 | {{kulpart|BBa_B0032|icon}}|| RBS || Ap || Available here || -80 ||
 |-align="center"
-| {{kulpart|BBa_J23110|icon}}|| promotor || Ap || Available here || -80 || Terug te vinden in de -20
+| {{kulpart|BBa_J23110|icon}}|| promotor || Ap || Available here || -80 || Can be found in -20 freezer
 |-align="center"
 | {{kulpart|BBa_I742106|icon}}|| RBS + Sam8 || A || send from Edinburgh || -20 ||
@@ Line 29: / Line 29: @@
 | {{kulpart|BBa_I742113|icon}}|| ech || Ap || received from Edinburgh || -20 ||
 |-align="center"
-| {{kulpart|BBa_K238008|icon}}|| VirA receptor ||  || primers ordered || || This is purified from Agrobacterium temefaciens
+| {{kulpart|BBa_K238008|icon}}|| VirA receptor ||  || primers ordered || || This is purified from Agrobacterium tumefaciens
 |-align="center"
 | {{kulpart|BBa_K238009|icon}}|| VirG activator ||  || primers ordered || || This is purified from Agrobacterium tumefaciens