"
Template:Team:KULeuven/4 September 2009/BlueLightReceptor
From 2009.igem.org
(Difference between revisions)
| Line 4: | Line 4: | ||
# A new restriction digest on {{kulpart|BBa_J61002}} and {{kulpart|BBa_K238013}} with EcoRI and SpeI was performed. | # A new restriction digest on {{kulpart|BBa_J61002}} and {{kulpart|BBa_K238013}} with EcoRI and SpeI was performed. | ||
# Gel extraction of these new digests. {{kulpart|BBa_J61002}} was nanodropped with a concentration of 28 ng/µl. this was ligated with {{kulpart|BBa_K238013}} (ligX 2) | # Gel extraction of these new digests. {{kulpart|BBa_J61002}} was nanodropped with a concentration of 28 ng/µl. this was ligated with {{kulpart|BBa_K238013}} (ligX 2) | ||
| - | # Electroporation of {{kulpart| | + | # Electroporation of {{kulpart|pSB3K3}} |
| Line 10: | Line 10: | ||
#Electroporation of ligX 1&2 | #Electroporation of ligX 1&2 | ||
| - | #There was no growth of cells after electroporation with {{kulpart| | + | #There was no growth of cells after electroporation with {{kulpart|pSB3K3}} |
=Sunday= | =Sunday= | ||
# There was good growth of the cells that were electroporated with ligX. 4 single colonies were selected and plated on Ap medium. This was all done in a dark room to avoid activation of the promotor. | # There was good growth of the cells that were electroporated with ligX. 4 single colonies were selected and plated on Ap medium. This was all done in a dark room to avoid activation of the promotor. | ||
Revision as of 14:36, 10 September 2009
- Gel extraction performed on the signals from 3/09
- Nanodrop: 14,7 ng/µl of with a 260/280 of approx 2,12
- Ligation with and (ligX 1)
- A new restriction digest on and with EcoRI and SpeI was performed.
- Gel extraction of these new digests. was nanodropped with a concentration of 28 ng/µl. this was ligated with (ligX 2)
- Electroporation of
Saturday
- Electroporation of ligX 1&2
- There was no growth of cells after electroporation with
Sunday
- There was good growth of the cells that were electroporated with ligX. 4 single colonies were selected and plated on Ap medium. This was all done in a dark room to avoid activation of the promotor.
