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Team:Imperial College London/M2/Genetic
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[[Image:m2.fgc1.jpg | 450px]] | [[Image:m2.fgc1.jpg | 450px]] | ||
[[Image:m2.fgc2.JPG | 300px]] | [[Image:m2.fgc2.JPG | 300px]] | ||
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| + | ==Elaboration== | ||
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| + | This genetic circuit consists of two parts. The first part deals with the production of colanic acid, while the second part deals with the production of trehalose. Colanic acid is being used to encapsulate our drug protein, protecting it from the harsh acidic environment of our gut. Trehalose will ensure the stability of the colanic acid coat. | ||
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| + | Genes | ||
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| + | Encapsulation is auto-induced when the concentration of glucose decreases below threshold. Initially, glucose concentration is high and this represses the CRP promoter. No transcription occurs and encapsulation is repressed. As E. coli bacteria grows, it consumes glucose, resulting in a decrease in glucose concentration until the threshold is reached. At this point, glucose will no longer be able to repress the CRP promoter. Colanic acid and trehalose would be produced. | ||
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Revision as of 15:51, 10 September 2009
Contents |
M2 Genetic Circuit
Video
Genetic circuit
Elaboration
This genetic circuit consists of two parts. The first part deals with the production of colanic acid, while the second part deals with the production of trehalose. Colanic acid is being used to encapsulate our drug protein, protecting it from the harsh acidic environment of our gut. Trehalose will ensure the stability of the colanic acid coat.
Genes
Encapsulation is auto-induced when the concentration of glucose decreases below threshold. Initially, glucose concentration is high and this represses the CRP promoter. No transcription occurs and encapsulation is repressed. As E. coli bacteria grows, it consumes glucose, resulting in a decrease in glucose concentration until the threshold is reached. At this point, glucose will no longer be able to repress the CRP promoter. Colanic acid and trehalose would be produced.
