Template:Team:KULeuven/7 September 2009/VanillinProduction

From 2009.igem.org

(Difference between revisions)
Line 8: Line 8:
| EF 2 || 105,2 || 1,96 || 1,66
| EF 2 || 105,2 || 1,96 || 1,66
|- align="center"
|- align="center"
-
| EF 3 || 95,1 || 12,05 || 0,98
+
| EF 3 || 95,1 || 2,05 || 0,98
|- align="center"
|- align="center"
| EF 4 || 52,7 || 2,06 || 0,97
| EF 4 || 52,7 || 2,06 || 0,97

Revision as of 16:08, 10 September 2009

  • Miniprep from the four colonies of EF ligation
    • Accidentally used double volumes of elution buffer on EF4
part concentration 260/280 260/230
EF 1 115,5 1,98 2,31
EF 2 105,2 1,96 1,66
EF 3 95,1 2,05 0,98
EF 4 52,7 2,06 0,97
  • Gel electrophoresis of restriction from Sam5 and Sam8 looked good
  • Purified and ligated the restriction from Sam5 and Sam8 overnight
    • Concentration for sam8 was barely enough for ligation...
part concentration 260/280 260/230
Sam5 10,1 2,11 0,04
Sam8 3,1 3,96 0,02
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