Template:Team:KULeuven/9 September 2009/BlueLightReceptor

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(Difference between revisions)
(New page: # FACS-ing the liquid cultures that were put under blue light on 8/09 gave no significant results. # motherplate for {{kulpart|BBa_J23101}} was made # liquid cultures for checking the lig...)
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# motherplate for {{kulpart|BBa_J23101}} was made
# motherplate for {{kulpart|BBa_J23101}} was made
# liquid cultures for checking the ligX were made.
# liquid cultures for checking the ligX were made.
 +
# LC with {{kulpart|BBa_J23101}} were miniprepped, nanodropped, restrictie digested and put on gel. the signals were good. the vector, pSB1A2, was cut from the gel and gel extracted. this was ligated with {{kulpart|BBa_K238013}}.
 +
nanodrop:
 +
{| border ="1" align="center"
 +
!| Part || concentration (ng/μl) || 260/280 λ ||
 +
|- align="center"
 +
| {{kulpart|BBa_J23101}}(1) || 141,8 || 1,94 ||
 +
|- align="center"
 +
| {{kulpart|BBa_J23101}}(2) || 139,5 || 1,99 ||
 +
|- align="center"
 +
| {{kulpart|BBa_J23101}}(3) || 177,5 || 1,95 ||
 +
|- align="center"
 +
| {{kulpart|BBa_J23101}}(4) || 141 || 1,95 ||
 +
|}
 +
gel extractie:
 +
|- align="center"
 +
| {{kulpart|BBa_J23101}} || 10,8 ||  ||

Revision as of 16:23, 10 September 2009

  1. FACS-ing the liquid cultures that were put under blue light on 8/09 gave no significant results.
  2. motherplate for was made
  3. liquid cultures for checking the ligX were made.
  4. LC with were miniprepped, nanodropped, restrictie digested and put on gel. the signals were good. the vector, pSB1A2, was cut from the gel and gel extracted. this was ligated with .

nanodrop:

Part concentration (ng/μl) 260/280 λ
(1) 141,8 1,94
(2) 139,5 1,99
(3) 177,5 1,95
(4) 141 1,95

gel extractie: |- align="center" | || 10,8 || ||

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